Genotypic characterization of textile dye degrading bacteria by RAPD, rep-PCR and PCR-RFLP of 16S rDNA studies

Dyes are xenobiotic compounds that are very recalcitrant against natural degradation processes. Biodegradation by microbial flora is an alternative treatment option other than the commonly employed physico-chemical methods to treat these toxic effluents. The aim of present study was to isolate and c...

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Bibliographic Details
Published in:International Journal of Biological Sciences and Technology 2012-01, Vol.4 (1), p.1-1
Main Authors: Soni, Anjali P, Parmar, Preeti R, Desai, Piyush V, Ranade, Shirish A
Format: Article
Language:English
Subjects:
Biodegradation
decolorization
Differentiation
DNA fingerprinting
Dyes
Effluents
Endonuclease
Genetic diversity
Polymerase chain reaction
Population studies
Pseudomonas
Random amplified polymorphic DNA
Restriction fragment length polymorphism
rRNA 16S
Soil
Soil microorganisms
Textile industry
Textiles
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Summary:Dyes are xenobiotic compounds that are very recalcitrant against natural degradation processes. Biodegradation by microbial flora is an alternative treatment option other than the commonly employed physico-chemical methods to treat these toxic effluents. The aim of present study was to isolate and characterize the effluent adapted indigenous bacteria from soil taken from sites contaminated due to the textile industry effluents. Four commonly used dyes are taken and simulated effluents are prepared on which the decolourization activity of the isolated strains is tested. Total ten bacterial strains were isolated from soil. Approximately 50% decolourisation with one strain on two dyes in 10 days was obtained. The actual composition and genetic diversity of natural field population was studied using different PCR fingerprinting methods such as RAPD, ERIC-PCR and 16SrDNA.PCR-RFLP. Isolates are characterized by RAPD and ERIC-PCR generated highly specific and reproducible pattern that enabled accurate strain differentiation. Dendrograms derived by RAPD and ERIC profiles showed that all strains could be divided into three groups. Cluster analysis of combined RFLP patterns obtained by three endonucleases revealed that mainly Pseudomonas species are adapted to the dye environment and utilized for the treatment of the textile effluent.
ISSN:0975-8704