Diversity of Bacterial Communities Associated with the Indian Ocean Sponge Tsitsikamma favus That Contains the Bioactive Pyrroloiminoquinones, Tsitsikammamine A and B

Tsitsikamma favus is a latrunculid sponge endemic to the coast of South Africa that produces unique pyrroloiminoquinones known as tsitsikammamines. Wakayin and makaluvamine A are structurally similar to the tsitsikammamines and are the only pyrroloiminoquinones isolated from a source other than Pori...

Full description

Saved in:
Bibliographic Details
Published in:Marine biotechnology (New York, N.Y.) N.Y.), 2012-12, Vol.14 (6), p.681-691
Main Authors: Walmsley, Tara A., Matcher, Gwynneth F., Zhang, Fan, Hill, Russell T., Davies-Coleman, Michael T., Dorrington, Rosemary A.
Format: Article
Language:English
Subjects:
Animals
Aquatic life
Bacteria
Betaproteobacteria - classification
Betaproteobacteria - isolation & purification
Betaproteobacteria - metabolism
Biodiversity
Biomedical and Life Sciences
Biotechnology
Clavelina
Defense mechanisms
Didymium
Engineering
Freshwater & Marine Ecology
Indian Ocean
Laboratories
Laboratory culture
Life Sciences
Marine
Marine biology
Metabolites
Microbial Consortia - physiology
Microbiology
Myxomycetes
Original Article
Pharmaceutical industry
Porifera
Porifera - microbiology
Pyrroles - metabolism
Pyrroloiminoquinones - metabolism
Quinolines - metabolism
Species Specificity
Studies
Zoology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Tsitsikamma favus is a latrunculid sponge endemic to the coast of South Africa that produces unique pyrroloiminoquinones known as tsitsikammamines. Wakayin and makaluvamine A are structurally similar to the tsitsikammamines and are the only pyrroloiminoquinones isolated from a source other than Porifera (namely a Fijian ascidian Clavelina sp. and a laboratory culture of the myxomycete Didymium bahiense , respectively). The source of the tsitsikammamines is hypothesised to be microbial, which could provide a means of overcoming the current supply problem. This study focuses on characterising the microbial diversity associated with T. favus . We have used denaturing gradient gel electrophoresis together with clonal and deep sequencing of microbial 16S rRNA gene amplicons to show that specimens of this sponge species contain a distinct and conserved microbial population, which is stable over time and is dominated by a unique Betaproteobacterium species.
ISSN:1436-2228
1436-2236
DOI:10.1007/s10126-012-9430-y