catA Gene in a Potential Corynebacterium Strain is Responsible for its Efficiency in Phenol Bioremoval

Out of 10 bacterial strains isolated out of wastewater of a pharmaceutical industry the most efficient bacterium, which could tolerate 5,000 mgl −1 of phenol in the minimal medium, was identified to be Corynebacterium sp.DST1 through 16S rDNA analysis. This strain could remove 99.4% of phenol from t...

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Bibliographic Details
Published in:Polycyclic aromatic compounds 2012-08, Vol.32 (4), p.423-438
Main Authors: DALAL, S., PANIGRAHI, D. P., RANDHAWA, G. S., DUBEY, R. C.
Format: Article
Language:English
Subjects:
16S rDNA
Bacteria
Bioremediation
catA
Corynebacterium
degenerate primers
Deoxyribonucleic acid
DNA
Enzymes
PCR
Pharmaceutical industry
phenol
sawdust
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Summary:Out of 10 bacterial strains isolated out of wastewater of a pharmaceutical industry the most efficient bacterium, which could tolerate 5,000 mgl −1 of phenol in the minimal medium, was identified to be Corynebacterium sp.DST1 through 16S rDNA analysis. This strain could remove 99.4% of phenol from the minimal medium containing 4,000 mgl −1 of phenol. The ortho catalytic pathway using the enzyme catechol 1, 2- dioxygenase was found to be the major pathway for phenol degradation in the bacterium. The gene for catechol 1, 2-dioxygenase (catA) could also be amplified using degenerate primers. Corynebacterium sp.DST1 cells were attached to sawdust and removal of phenol from industrial effluents was evaluated. About 92.7% of phenol could be removed from the effluent of a heavy electrical industry. The results of this study not only suggest the potentiality of the strain to remove phenol efficiently from crude industrial effluents but also display a cheap method of bioremediation using sawdust.
ISSN:1040-6638
1563-5333
DOI:10.1080/10406638.2011.645267