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Absolute intensity and molecular weight determination of samples containing small amounts of impurities

Absolute intensity calibration in static light scattering experiments means to bring the scattered intensity detected as number of counts per time by a photomultiplier to an absolute scale cm−1. For this purpose a reference standard (e.g. toluene or water), from which the absolute scattering is know...

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Bibliographic Details
Published in:Macromolecular symposia. 2000-12, Vol.162 (1), p.81-86
Main Authors: Lindner, Helmut, Glatter, Otto
Format: Article
Language:English
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Summary:Absolute intensity calibration in static light scattering experiments means to bring the scattered intensity detected as number of counts per time by a photomultiplier to an absolute scale cm−1. For this purpose a reference standard (e.g. toluene or water), from which the absolute scattering is known, can be used. Having small particles compared to the wavelength e.g. proteins or micelles mixed with a small amount of large aggregates one is confronted with the problem that these impurities normally contain only a very small part of the sample mass, but contribute strongly to the measured signal. To separate the scattered intensity from the large impurities or aggregates we use the additional information obtainable from a dynamic light scattering measurement. The areas in the intensity distribution function, which can be calculated by inverse Laplace transformation of the field correlation function, are directly related to the intensity of the particular populations. In this contribution we show with simulations that this procedure works well in a quite large range of relative intensities and sizes.
ISSN:1022-1360
1521-3900
DOI:10.1002/1521-3900(200012)162:1<81::AID-MASY81>3.0.CO;2-6