Novel nucleoside analogue FNC is effective against both wild-type and lamivudine-resistant HBV clinical isolates

• Published in: Antiviral therapy 2012-01, Vol.17 (8), p.1593-1599
• Main Authors: Zhou, Yubing, Zhang, Yan, Yang, Xiaoang, Zhao, Jing, Zheng, Liyun, Sun, Changyu, Jiang, Jinhua, Yang, Qinghua, Wang, Qingduan, Chang, Junbiao
• Format: Article
• Language: English
• Subjects: Adult; Antibiotics. Antiinfectious agents. Antiparasitic agents; Antiviral activity; Antiviral agents; Antiviral Agents - pharmacology; Antiviral Agents - toxicity; Azides - pharmacology; Azides - toxicity; Biological and medical sciences; Cell Line; Cell Survival - drug effects; Clinical isolates; Deoxycytidine - analogs & derivatives; Deoxycytidine - pharmacology; Deoxycytidine - toxicity; Drug resistance; Drug Resistance, Viral - genetics; Expression vectors; Genotype; Hep G2 Cells; Hepatitis B virus; Hepatitis B virus - drug effects; Hepatitis B virus - genetics; Hepatitis B virus - isolation & purification; Humans; Infection; Lamivudine; Male; Medical sciences; nucleoside analogs; Pharmacology. Drug treatments; Polymerase chain reaction; Public health; Replication; Transfection; Virus Replication - drug effects
• ISSN: 1359-6535; 2040-2058
• DOI: 10.3851/IMP2292

HBV infection causes major public health problems worldwide. The clinical limitation of current antiviral drugs for HBV, such as lamivudine, is causing rapid emergence of drug-resistant viral strains during prolonged antiviral therapy. Therefore, new antiviral drugs are urgently needed to prevent or delay the selection of drug-resistant HBV mutants. A novel cytidine analogue, FNC (2'-deoxy-2'-β-fluoro-4'-azidocytidine), was recently shown to strongly inhibit human HBV and duck HBV (DHBV) replication in vitro and in vivo, respectively. The present study was designed to evaluate the in vitro antiviral activity of FNC against clinical wild-type and lamivudine-resistant HBV isolates in transiently transfected cells. HBV DNA was extracted from serum samples collected both before lamivudine therapy and at the time of viral breakthrough and was amplified by PCR. The amplicon was cloned into a novel expression vector, pHY106, which can initiate the intracellular HBV replication cycle after cell transfection. Following transfection of the cloned amplicon into HepG2 cells, a drug susceptibility assay was performed. Quantitative real-time PCR was used for determining the amount of intracellular HBV DNA, and the effective concentration required to reduce HBV replication by 50% (EC(50)) was calculated. FNC inhibited the replication of both wild-type and lamivudine-resistant HBV clinical isolates in a dose-dependent manner, with mean ±SD EC(50) values of 0.12 ±0.01 μM and 0.27 ±0.01 μM, respectively. FNC is a potential antiviral agent against both wild-type and lamivudine-resistant HBV clinical isolates, and therefore deserves further evaluation for the treatment of HBV infection.