A simple HPLC method for the comprehensive analysis of cis/trans (Z/E) geometrical isomers of carotenoids for nutritional studies

• Published in: Food chemistry 2013-06, Vol.138 (2-3), p.1341-1350
• Main Authors: Melendez-Martinez, Antonio J., Stinco, Carla M., Liu, Chun, Wang, Xiang-Dong
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Carotenoids; Carotenoids - chemistry; Carotenoids - metabolism; Chromatography, High Pressure Liquid - methods; cis/trans; Feeding. Feeding behavior; Ferrets; Food industries; Fundamental and applied biological sciences. Psychology; Geometrical isomers; Humans; Lung - chemistry; Lung - metabolism; Lycopene; Phytoene; Phytofluene; Plasma; Serum; Stereoisomerism; Vertebrates: anatomy and physiology, studies on body, several organs or systems
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2012.10.067

► Forty eight isomers of major human carotenoids were separated by a new HPLC method. ► This is to the best of our knowledge the highest number separated so far. ► Twenty-six different carotenoid isomers were detected in human plasma and ferret lung. ► UV/vis data of isomers of the carotenes phytoene and phytofluene are provided. Geometrical isomers of carotenoids behave differently in aspects like stability towards oxidants, bioavailability, vitamin A activity and specificity for enzymes. The availability of HPLC methods for their detailed profiling is therefore advisable to expand our knowledge on their metabolism and biological role. In this paper the development of a methodology to determine the highest number of geometrical isomers of major carotenoids in humans (phytoene, phytofluene, lutein, zeaxanthin, β-cryptoxanthin, α-carotene, β-carotene and lycopene) is described. To assess its usefulness with biological samples both postprandial human plasma and lung samples from ferrets were analysed. Up to 48 isomers of the main human carotenoids were separated in 62min. This is to the best of our knowledge the report of the highest number of carotenoid geometrical isomers separated with a HPLC method. Twenty-six different carotenoid isomers were readily detected in the biological samples.