Transcriptome profiling of genes induced by salicylic acid and methyl jasmonate in Polygonum minus

The importance of plant secondary metabolites for both mankind and the plant itself has long been established. However, despite extensive research on plant secondary metabolites, plant secondary metabolism and its regulation still remained poorly characterized. In this present study, cDNA-amplified...

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Bibliographic Details
Published in:Molecular biology reports 2013-03, Vol.40 (3), p.2231-2241
Main Authors: Ee, Su-Fang, Oh, Ji-Min, Mohd Noor, Normah, Kwon, Taek-Ryoun, Mohamed-Hussein, Zeti-Azura, Ismail, Ismanizan, Zainal, Zamri
Format: Article
Language:English
Subjects:
Acetates - pharmacology
Animal Anatomy
Animal Biochemistry
Biomedical and Life Sciences
Biosynthesis
Cyclopentanes - pharmacology
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Plant - drug effects
Histology
Life Sciences
Metabolites
Molecular biology
Molecular Sequence Annotation
Molecular Sequence Data
Morphology
Oxylipins - pharmacology
Phenotype
Plant Growth Regulators - pharmacology
Plant Leaves
Polygonum
Polygonum - drug effects
Polygonum - genetics
Polymorphism
Salicylic Acid - pharmacology
Stress response
Transcription, Genetic
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Summary:The importance of plant secondary metabolites for both mankind and the plant itself has long been established. However, despite extensive research on plant secondary metabolites, plant secondary metabolism and its regulation still remained poorly characterized. In this present study, cDNA-amplified fragment length polymorphism (cDNA-AFLP) transcript profiling was applied to generate the expression profiles of Polygonum minus in response to salicylic acid (SA) and methyl jasmonate (MeJA) elicitations. This study reveals two different sets of genes induced by SA and MeJA, respectively where stress-related genes were proved to lead to the expression of genes involved in plant secondary metabolite biosynthetic pathways. A total of 98 transcript-derived fragments (TDFs) were up-regulated, including 46 from SA-treated and 52 from MeJA-treated samples. The cDNA-AFLP transcripts generated using 64 different Mse 1/ Taq 1 primer combinations showed that treatments with SA and MeJA induced genes mostly involved in scavenging reactive oxygen species, including zeaxanthin epoxidase, cytosolic ascorbate peroxidase 1 and peroxidase. Of these stress-related genes, 15 % of other annotated TDFs are involved mainly in secondary metabolic processes where among these, two genes encoding (+)-delta cadinene synthase and cinnamoyl-CoA reductase were highlighted.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-012-2286-4