The interaction between Trypanosoma rangeli and the nitrophorins in the salivary glands of the triatomine Rhodnius prolixus (Hemiptera; Reduviidae)

The parasite Trypanosoma rangeli develops in the intestinal tract of triatomines and, particularly in species of the genus Rhodnius, invades the hemolymph and salivary glands, where subsequent metacyclogenesis takes place. Many aspects of the interaction between T. rangeli and triatomines are still...

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Bibliographic Details
Published in:Insect biochemistry and molecular biology 2013-03, Vol.43 (3), p.229-236
Main Authors: Paim, Rafaela M.M., Pereira, Marcos H., Araújo, Ricardo N., Gontijo, Nelder F., Guarneri, Alessandra A.
Format: Article
Language:English
Subjects:
actin
Animals
biosynthesis
blood
epimastigotes
essential genes
Hemeproteins - genetics
Hemeproteins - metabolism
Hemiptera
hemolymph
Host-Parasite Interactions
insects
instars
intestines
molting
Nitrophorins
nutrients
nymphs
parasites
quantitative polymerase chain reaction
Reduviidae
Rhodnius
Rhodnius - genetics
Rhodnius - metabolism
Rhodnius - parasitology
Rhodnius prolixus
ribosomal RNA
RNA Interference
saliva
Saliva - metabolism
Salivary glands
Salivary Glands - metabolism
Salivary Glands - parasitology
Salivary Proteins and Peptides - genetics
Salivary Proteins and Peptides - metabolism
Trypanosoma rangeli
Trypanosoma rangeli - physiology
trypomastigotes
tubulin
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Summary:The parasite Trypanosoma rangeli develops in the intestinal tract of triatomines and, particularly in species of the genus Rhodnius, invades the hemolymph and salivary glands, where subsequent metacyclogenesis takes place. Many aspects of the interaction between T. rangeli and triatomines are still unclear, especially concerning the development of the parasite in the salivary glands and how the parasite interacts with the saliva. In this work, we describe new findings on the process of T. rangeli infection of the salivary glands and the impact of infection on the saliva composition. To ensure a complete infection (intestinal tract, hemolymph and salivary glands), 3rd instar Rhodnius prolixus nymphs were fed on blood containing T. rangeli epimastigotes using an artificial feeder. After molt to the 4th instar, the nymphs were inoculated with epimastigotes in the hemolymph. The results showed that the flagellates started to invade the salivary glands by the 7th day after the injection. The percentage of trypomastigotes inside the salivary glands continuously increased until the 25th day, at which time the trypomastigotes were more than 95% of the T. rangeli forms present. The salivary contents from T. rangeli-infected insects showed a pH that was significantly more acidic (
ISSN:0965-1748
1879-0240
DOI:10.1016/j.ibmb.2012.12.011