Expression patterns and potential roles of SIRT1 in human medulloblastoma cells in vivo and in vitro

• Published in: Neuropathology 2013-02, Vol.33 (1), p.7-16
• Main Authors: Ma, Jing-Xin, Li, Hong, Chen, Xiao-Ming, Yang, Xiang-Hong, Wang, Qian, Wu, Mo-Li, Kong, Qing-You, Li, Zai-Xia, Liu, Jia
• Format: Article
• Language: English
• Subjects: Apoptosis; Apoptosis - drug effects; Apoptosis - genetics; Cell Cycle - drug effects; Cell Cycle - genetics; Cell Differentiation - drug effects; Cell Differentiation - genetics; cell growth; Cell Line, Tumor; Cell proliferation; Cell survival; Cerebellar Neoplasms - genetics; Cerebellar Neoplasms - metabolism; Cerebellar Neoplasms - pathology; Cerebellum; Cerebellum - drug effects; Cerebellum - metabolism; Cerebellum - pathology; Data processing; Differentiation; G1 phase; gene expression; Granule cells; Histone deacetylase; Humans; Medulloblastoma; Medulloblastoma - genetics; Medulloblastoma - metabolism; Medulloblastoma - pathology; Nervous system diseases; Neurogenetics; Neurons - drug effects; Neurons - metabolism; Neurons - pathology; Niacinamide - pharmacology; nicotinamide; Prognosis; Promoters; siRNA; SIRT1; SIRT1 inhibitor; SIRT1 protein; Sirtuin 1 - genetics; Sirtuin 1 - metabolism; Sirtuins; Transfection
• ISSN: 0919-6544; 1440-1789
• DOI: 10.1111/j.1440-1789.2012.01318.x

Medulloblastoma is a primitive neuroectodermal tumor, which originates in the cerebellum, presumably due to the alterations of some neurogenetic elements. Sirtuin 1 (SIRT1), a class III histone deacetylase (HDAC), regulates differentiation of neuronal stem cells but its status in medulloblastomas remains largely unknown. The current study aimed to address this issue by checking SIRT1 expression in noncancerous cerebellar tissues, medulloblastoma tissues and established cell lines. The roles of SIRT1 in proliferation and survival of UW228‐3 medulloblastoma cells were analyzed by SIRT1 small interfering RNA (siRNA) transfection and SIRT1 inhibitor nicotinamide treatment. The results revealed that the frequency of SIRT1 expression in medulloblastoma tissues was 64.17% (77/120), while only one out of seven tumor‐surrounding noncancerous cerebellar tissues showed restricted SIRT1 expression in the cells within the granule layer. Of the three morphological subtypes, the rates of SIRT1 detection in the large cell/anaplastic cell (79.07%; 34/43) and the classic medulloblastomas (60.29%; 41/68) are higher than that (22.22%; 2/9) in nodular/desmoplastic medulloblastomas (P