New oxidative decomposition mechanism of estradiol through the structural characterization of a minute impurity and its degradants

• Published in: Journal of pharmaceutical and biomedical analysis 2013-05, Vol.78-79, p.183-189
• Main Authors: Béni, Zoltán, Háda, Viktor, Varga, Emese, Mahó, Sándor, Aranyi, Antal, Szántay, Csaba
• Format: Article
• Language: English
• Subjects: acetates; active ingredients; Chromatography, High Pressure Liquid; Drug Contamination; drug formulations; epoxides; Estradiol; Estradiol - chemistry; high performance liquid chromatography; Impurity; ionization; LC–MS/MS; LC–NMR; NMR; nuclear magnetic resonance spectroscopy; Oxidation; Oxidation-Reduction; Tandem Mass Spectrometry
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2013.02.015

[Display omitted] ► A low level oxidative degradant of estradiol detected in a formulation was structurally characterized. ► A new oxidative degradation mechanism of estradiol is proposed. ► Literature ambiguities were corrected and rationalized. Herein we discuss the structure elucidation of a labile estradiol-related degradant, X1. X1 was detected at Gedeon Richter as an unknown trace impurity in a pharmaceutical formulation containing estradiol (1a) and norethisterone acetate (NA) as active ingredients. The structural identification of X1 proved to be an unusually complex task involving an initial structural hypothesis based on some limited analytical data (UV) obtained from the formulation, synthetic work targeting the proposed structure, chromatographic enrichment from the synthetic reaction mixture, (HPLC)–MS and MS–MS studies of the formulation and of samples from the synthesis using almost all available ionization modes, preparative LC enrichment, and the complementary use of off-line and on-line NMR techniques. Based on these results, X1 was finally characterized as a new oxidative product of estradiol, containing an epoxy function over the C9–C10 bond. During the structure determination of X1 its secondary and tertiary decomposition products were also identified as a new secoepoxy (6) and a known seco derivative (5a) of estradiol, respectively. On this basis a new oxidative decomposition mechanism of estradiol and its analogues could be proposed. A generalization of the mechanism of this pathway can more readily explain the formation of some oxidative secosteroid degradants than the mechanism proposed earlier in the literature.