Understanding microstructural changes of starch during atmospheric and vacuum heating in water and oil through online in situ vacuum hot-stage microscopy

• Published in: Innovative food science & emerging technologies 2013-01, Vol.17, p.135-143
• Main Authors: Ovalle, N., Cortés, P., Bouchon, P.
• Format: Article
• Language: English
• Subjects: Atmospheric frying; Atmospherics; Fabricated products; Formulated products; Frying; Heating; Hot-stage microscopy; Image analysis; Low vacuum; Microscopes; Microscopy; Microstructure; Process miniaturization; Starch gelatinization; Starches; Vacuum frying
• ISSN: 1466-8564; 1878-5522
• DOI: 10.1016/j.ifset.2012.11.006

Starch gelatinization is a critical micro-scale phenomenon occurring during frying, which gets particular relevance during vacuum frying, where minimum conditions need to be fulfilled to get the required structure. The objective of this research was to understand the effect of water accessibility, temperature and vacuum level, in starch microstructural changes during heating in water and oil, in situ and in real time, using vacuum hot-stage microscopy. Potato starch was heated in water or in a pre-gelatinized corn starch solution, at different pressures (atmospheric, 6.5kPa, 30kPa). Also, a gluten–starch matrix immersed in oil was followed under the microscope. An increase in the heating rate as well as water inaccessibility increased the on-set gelatinization temperature. Under high vacuum no changes were observed, whereas under low vacuum, partial gelatinization occurred. Overall, this microstructural approach may help in explaining the high oil absorption of vacuum-fried formulated starchy products reported in previous studies. Vacuum frying is a novel and promising technique that allows obtaining fried products with a low amount of oil, making them healthier than atmospheric fried ones. So far most of the studies have focused on agricultural products, such as potatoes, which are already structured by nature, without focusing on fabricated/restructured products. This imposes a challenge since minimum conditions must be ensured to form structure during processing in this product category. In fact, vacuum fried restructured products have shown to increase their oil content compared to their atmospheric counterpart, probably due to lack of structure formation during processing. From this perspective, the understanding of the main microstructural changes of starch in formulated products during processing under atmospheric or vacuum conditions is of interest. Since, most of the structural changes occur at the micro-scale, process miniaturization using vacuum hot-stage microscopy seems to be a good approach. In accordance, the objective of this research was to understand the effect of water availability, temperature and vacuum level, in starch microstructural changes during heating in water and oil, in situ and in real time. Potato starch was heated in excess of water or in a pre-gelatinized corn starch solution, at different pressures. Also, a gluten–starch matrix immersed in oil was followed under the microscope. We found that an increase in the heating rat