Disulfide Biochemistry in 2-Cys Peroxiredoxin: Requirement of Glu50 and Arg146 for the Reduction of Yeast Tsa1 by Thioredoxin

• Published in: Journal of molecular biology 2012-11, Vol.424 (1-2), p.28-41
• Main Authors: Tairum, Carlos A., de Oliveira, Marcos A., Horta, Bruno B., Zara, Fernando J., Netto, Luis E.S.
• Format: Article
• Language: English
• Subjects: antioxidants; Arginine - metabolism; Chromatography, Gel; crystal structure; cysteine; Disulfides - metabolism; Glutamic Acid - metabolism; hydrogen peroxide; hydroperoxides; Models, Molecular; Molecular Sequence Data; mutants; oxidation; Oxidation-Reduction; peroxidase; Peroxidases - chemistry; Peroxidases - metabolism; peroxiredoxin; Peroxiredoxins - metabolism; protein–protein interactions; Saccharomyces cerevisiae; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - chemistry; Saccharomyces cerevisiae Proteins - metabolism; signal transduction; thioredoxin; Thioredoxins - metabolism; yeasts
• ISSN: 0022-2836; 1089-8638
• DOI: 10.1016/j.jmb.2012.09.008

2-Cys peroxiredoxin (Prx) enzymes are ubiquitously distributed peroxidases that make use of a peroxidatic cysteine (CysP) to decompose hydroperoxides. A disulfide bond is generated as a consequence of the partial unfolding of the α-helix that contains CysP. Therefore, during its catalytic cycle, 2-Cys Prx alternates between two states, locally unfolded and fully folded. Tsa1 (thiol‐specific antioxidant protein 1 from yeast) is by far the most abundant Cys-based peroxidase in Saccharomyces cerevisiae. In this work, we present the crystallographic structure at 2.8Å resolution of Tsa1C47S in the decameric form [(α2)5] with a DTT molecule bound to the active site, representing one of the few available reports of a 2-Cys Prx (AhpC-Prx1 subfamily) (AhpC, alkyl hydroperoxide reductase subunit C) structure that incorporates a ligand. The analysis of the Tsa1C47S structure indicated that Glu50 and Arg146 participate in the stabilization of the CysP α-helix. As a consequence, we raised the hypothesis that Glu50 and Arg146 might be relevant to the CysP reactivity. Therefore, Tsa1E50A and Tsa1R146Q mutants were generated and were still able to decompose hydrogen peroxide, presenting a second-order rate constant in the range of 106M−1s−1. Remarkably, although Tsa1E50A and Tsa1R146Q were efficiently reduced by the low-molecular-weight reductant DTT, these mutants displayed only marginal thioredoxin (Trx)-dependent peroxidase activity, indicating that Glu50 and Arg146 are important for the Tsa1–Trx interaction. These results may impact the comprehension of downstream events of signaling pathways that are triggered by the oxidation of critical Cys residues, such as Trx. [Display omitted] ► 2-Cys Prxs are key enzymes in the peroxide metabolism. ► Tsa1 from S.cerevisiae is its main thiol-dependent peroxidase. ► Elucidation of the crystal structure of Tsa1 bound to a ligand (DTT). ► Glu50 and Arg146 stabilize Tsa1 in fully folded state. ► Glu50, Arg146 and Cys170 are needed for the reduction of Tsa1 by Trx.