Metagenomic insights into chlorination effects on microbial antibiotic resistance in drinking water

This study aimed to investigate the chlorination effects on microbial antibiotic resistance in a drinking water treatment plant. Biochemical identification, 16S rRNA gene cloning and metagenomic analysis consistently indicated that Proteobacteria were the main antibiotic resistant bacteria (ARB) dom...

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Bibliographic Details
Published in:Water research (Oxford) 2013-01, Vol.47 (1), p.111-120
Main Authors: Shi, Peng, Jia, Shuyu, Zhang, Xu-Xiang, Zhang, Tong, Cheng, Shupei, Li, Aimin
Format: Article
Language:English
Subjects:
Abundance
Anti-Bacterial Agents - pharmacology
Antibiotic resistance genes
Antibiotics
Applied sciences
Bacteria
Bacteria - genetics
Chlorination
Chlorine disinfection
Cloning
Disinfection
Drinking water
Drinking Water - microbiology
Drinking water and swimming-pool water. Desalination
Drug Resistance, Bacterial
Exact sciences and technology
Genes
Halogenation
Metagenomic analysis
Microorganisms
Mobile genetic elements
Pollution
Water Microbiology
Water Purification - methods
Water treatment and pollution
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Summary:This study aimed to investigate the chlorination effects on microbial antibiotic resistance in a drinking water treatment plant. Biochemical identification, 16S rRNA gene cloning and metagenomic analysis consistently indicated that Proteobacteria were the main antibiotic resistant bacteria (ARB) dominating in the drinking water and chlorine disinfection greatly affected microbial community structure. After chlorination, higher proportion of the surviving bacteria was resistant to chloramphenicol, trimethoprim and cephalothin. Quantitative real-time PCRs revealed that sulI had the highest abundance among the antibiotic resistance genes (ARGs) detected in the drinking water, followed by tetA and tetG. Chlorination caused enrichment of ampC, aphA2, blaTEM-1, tetA, tetG, ermA and ermB, but sulI was considerably removed (p 
ISSN:0043-1354
1879-2448
DOI:10.1016/j.watres.2012.09.046