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Metagenomic insights into chlorination effects on microbial antibiotic resistance in drinking water
This study aimed to investigate the chlorination effects on microbial antibiotic resistance in a drinking water treatment plant. Biochemical identification, 16S rRNA gene cloning and metagenomic analysis consistently indicated that Proteobacteria were the main antibiotic resistant bacteria (ARB) dom...
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| Published in: | Water research (Oxford) 2013-01, Vol.47 (1), p.111-120 |
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| cites | cdi_FETCH-LOGICAL-c425t-4d65362b4c878b2f0a44c02b6fc522dcf983b929cfbe5add30d3705d465db8483 |
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| creator | Shi, Peng Jia, Shuyu Zhang, Xu-Xiang Zhang, Tong Cheng, Shupei Li, Aimin |
| description | This study aimed to investigate the chlorination effects on microbial antibiotic resistance in a drinking water treatment plant. Biochemical identification, 16S rRNA gene cloning and metagenomic analysis consistently indicated that Proteobacteria were the main antibiotic resistant bacteria (ARB) dominating in the drinking water and chlorine disinfection greatly affected microbial community structure. After chlorination, higher proportion of the surviving bacteria was resistant to chloramphenicol, trimethoprim and cephalothin. Quantitative real-time PCRs revealed that sulI had the highest abundance among the antibiotic resistance genes (ARGs) detected in the drinking water, followed by tetA and tetG. Chlorination caused enrichment of ampC, aphA2, blaTEM-1, tetA, tetG, ermA and ermB, but sulI was considerably removed (p |
| doi_str_mv | 10.1016/j.watres.2012.09.046 |
| format | article |
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► Metagenomic analysis reveals prevalence of antibiotic resistance genes in drinking water. ► Chlorination helps to concentrate antibiotic resistance genes in drinking water. ► Pipeline transportation can alleviate antibiotic resistance in the chlorinated water.</description><identifier>ISSN: 0043-1354</identifier><identifier>EISSN: 1879-2448</identifier><identifier>DOI: 10.1016/j.watres.2012.09.046</identifier><identifier>PMID: 23084468</identifier><identifier>CODEN: WATRAG</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Abundance ; Anti-Bacterial Agents - pharmacology ; Antibiotic resistance genes ; Antibiotics ; Applied sciences ; Bacteria ; Bacteria - genetics ; Chlorination ; Chlorine disinfection ; Cloning ; Disinfection ; Drinking water ; Drinking Water - microbiology ; Drinking water and swimming-pool water. Desalination ; Drug Resistance, Bacterial ; Exact sciences and technology ; Genes ; Halogenation ; Metagenomic analysis ; Microorganisms ; Mobile genetic elements ; Pollution ; Water Microbiology ; Water Purification - methods ; Water treatment and pollution</subject><ispartof>Water research (Oxford), 2013-01, Vol.47 (1), p.111-120</ispartof><rights>2012 Elsevier Ltd</rights><rights>2014 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-4d65362b4c878b2f0a44c02b6fc522dcf983b929cfbe5add30d3705d465db8483</citedby><cites>FETCH-LOGICAL-c425t-4d65362b4c878b2f0a44c02b6fc522dcf983b929cfbe5add30d3705d465db8483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26673783$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23084468$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shi, Peng</creatorcontrib><creatorcontrib>Jia, Shuyu</creatorcontrib><creatorcontrib>Zhang, Xu-Xiang</creatorcontrib><creatorcontrib>Zhang, Tong</creatorcontrib><creatorcontrib>Cheng, Shupei</creatorcontrib><creatorcontrib>Li, Aimin</creatorcontrib><title>Metagenomic insights into chlorination effects on microbial antibiotic resistance in drinking water</title><title>Water research (Oxford)</title><addtitle>Water Res</addtitle><description>This study aimed to investigate the chlorination effects on microbial antibiotic resistance in a drinking water treatment plant. Biochemical identification, 16S rRNA gene cloning and metagenomic analysis consistently indicated that Proteobacteria were the main antibiotic resistant bacteria (ARB) dominating in the drinking water and chlorine disinfection greatly affected microbial community structure. After chlorination, higher proportion of the surviving bacteria was resistant to chloramphenicol, trimethoprim and cephalothin. Quantitative real-time PCRs revealed that sulI had the highest abundance among the antibiotic resistance genes (ARGs) detected in the drinking water, followed by tetA and tetG. Chlorination caused enrichment of ampC, aphA2, blaTEM-1, tetA, tetG, ermA and ermB, but sulI was considerably removed (p < 0.05). Metagenomic analysis confirmed that drinking water chlorination could concentrate various ARGs, as well as of plasmids, insertion sequences and integrons involved in horizontal transfer of the ARGs. Water pipeline transportation tended to reduce the abundance of most ARGs, but various ARB and ARGs were still present in the tap water, which deserves more public health concerns. The results highlighted prevalence of ARB and ARGs in chlorinated drinking water and this study might be technologically useful for detecting the ARGs in water environments.
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► Metagenomic analysis reveals prevalence of antibiotic resistance genes in drinking water. ► Chlorination helps to concentrate antibiotic resistance genes in drinking water. ► Pipeline transportation can alleviate antibiotic resistance in the chlorinated water.</description><subject>Abundance</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotic resistance genes</subject><subject>Antibiotics</subject><subject>Applied sciences</subject><subject>Bacteria</subject><subject>Bacteria - genetics</subject><subject>Chlorination</subject><subject>Chlorine disinfection</subject><subject>Cloning</subject><subject>Disinfection</subject><subject>Drinking water</subject><subject>Drinking Water - microbiology</subject><subject>Drinking water and swimming-pool water. Desalination</subject><subject>Drug Resistance, Bacterial</subject><subject>Exact sciences and technology</subject><subject>Genes</subject><subject>Halogenation</subject><subject>Metagenomic analysis</subject><subject>Microorganisms</subject><subject>Mobile genetic elements</subject><subject>Pollution</subject><subject>Water Microbiology</subject><subject>Water Purification - methods</subject><subject>Water treatment and pollution</subject><issn>0043-1354</issn><issn>1879-2448</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqFkUuLFDEUhYMoTtv6D0RqI7ip8uZRqdRGkEEdYcSNrkOePWmrK2OSVvz33qFbZ6erXMh3knPPIeQ5hYECla_3w0_TSqgDA8oGmAcQ8gHZUDXNPRNCPSQbAMF7ykdxQZ7UugcAxvj8mFwwDkoIqTbEfQrN7MKaD8l1aa1pd9MqDi137mbJJa2mpbx2Icbg8AZHJEu2ySydWVuyKTeUopFUm1ldQHHnUfctrbsOLYbylDyKZqnh2fnckq_v3325vOqvP3_4ePn2uneCja0XXo5cMiucmpRlEYwQDpiV0Y2MeRdnxe3MZhdtGI33HDyfYPRCjt4qofiWvDq9e1vy92OoTR9SdWFZzBrysWrKmRop8En8H2UMRjkrzGtLxAnFrWstIerbkg6m_NIU9F0Teq9PTei7JjTMGptA2YvzD0d7CP6v6E_0CLw8A6Y6s8SC4aV6z0k58Ulx5N6cuIDR_Uih6OpSwKB9KliJ9jn928lv-9OqSg</recordid><startdate>20130101</startdate><enddate>20130101</enddate><creator>Shi, Peng</creator><creator>Jia, Shuyu</creator><creator>Zhang, Xu-Xiang</creator><creator>Zhang, Tong</creator><creator>Cheng, Shupei</creator><creator>Li, Aimin</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>KR7</scope></search><sort><creationdate>20130101</creationdate><title>Metagenomic insights into chlorination effects on microbial antibiotic resistance in drinking water</title><author>Shi, Peng ; Jia, Shuyu ; Zhang, Xu-Xiang ; Zhang, Tong ; Cheng, Shupei ; Li, Aimin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-4d65362b4c878b2f0a44c02b6fc522dcf983b929cfbe5add30d3705d465db8483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Abundance</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotic resistance genes</topic><topic>Antibiotics</topic><topic>Applied sciences</topic><topic>Bacteria</topic><topic>Bacteria - genetics</topic><topic>Chlorination</topic><topic>Chlorine disinfection</topic><topic>Cloning</topic><topic>Disinfection</topic><topic>Drinking water</topic><topic>Drinking Water - microbiology</topic><topic>Drinking water and swimming-pool water. Desalination</topic><topic>Drug Resistance, Bacterial</topic><topic>Exact sciences and technology</topic><topic>Genes</topic><topic>Halogenation</topic><topic>Metagenomic analysis</topic><topic>Microorganisms</topic><topic>Mobile genetic elements</topic><topic>Pollution</topic><topic>Water Microbiology</topic><topic>Water Purification - methods</topic><topic>Water treatment and pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shi, Peng</creatorcontrib><creatorcontrib>Jia, Shuyu</creatorcontrib><creatorcontrib>Zhang, Xu-Xiang</creatorcontrib><creatorcontrib>Zhang, Tong</creatorcontrib><creatorcontrib>Cheng, Shupei</creatorcontrib><creatorcontrib>Li, Aimin</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Civil Engineering Abstracts</collection><jtitle>Water research (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shi, Peng</au><au>Jia, Shuyu</au><au>Zhang, Xu-Xiang</au><au>Zhang, Tong</au><au>Cheng, Shupei</au><au>Li, Aimin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metagenomic insights into chlorination effects on microbial antibiotic resistance in drinking water</atitle><jtitle>Water research (Oxford)</jtitle><addtitle>Water Res</addtitle><date>2013-01-01</date><risdate>2013</risdate><volume>47</volume><issue>1</issue><spage>111</spage><epage>120</epage><pages>111-120</pages><issn>0043-1354</issn><eissn>1879-2448</eissn><coden>WATRAG</coden><abstract>This study aimed to investigate the chlorination effects on microbial antibiotic resistance in a drinking water treatment plant. Biochemical identification, 16S rRNA gene cloning and metagenomic analysis consistently indicated that Proteobacteria were the main antibiotic resistant bacteria (ARB) dominating in the drinking water and chlorine disinfection greatly affected microbial community structure. After chlorination, higher proportion of the surviving bacteria was resistant to chloramphenicol, trimethoprim and cephalothin. Quantitative real-time PCRs revealed that sulI had the highest abundance among the antibiotic resistance genes (ARGs) detected in the drinking water, followed by tetA and tetG. Chlorination caused enrichment of ampC, aphA2, blaTEM-1, tetA, tetG, ermA and ermB, but sulI was considerably removed (p < 0.05). Metagenomic analysis confirmed that drinking water chlorination could concentrate various ARGs, as well as of plasmids, insertion sequences and integrons involved in horizontal transfer of the ARGs. Water pipeline transportation tended to reduce the abundance of most ARGs, but various ARB and ARGs were still present in the tap water, which deserves more public health concerns. The results highlighted prevalence of ARB and ARGs in chlorinated drinking water and this study might be technologically useful for detecting the ARGs in water environments.
[Display omitted]
► Metagenomic analysis reveals prevalence of antibiotic resistance genes in drinking water. ► Chlorination helps to concentrate antibiotic resistance genes in drinking water. ► Pipeline transportation can alleviate antibiotic resistance in the chlorinated water.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>23084468</pmid><doi>10.1016/j.watres.2012.09.046</doi><tpages>10</tpages></addata></record> |
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| subjects | Abundance Anti-Bacterial Agents - pharmacology Antibiotic resistance genes Antibiotics Applied sciences Bacteria Bacteria - genetics Chlorination Chlorine disinfection Cloning Disinfection Drinking water Drinking Water - microbiology Drinking water and swimming-pool water. Desalination Drug Resistance, Bacterial Exact sciences and technology Genes Halogenation Metagenomic analysis Microorganisms Mobile genetic elements Pollution Water Microbiology Water Purification - methods Water treatment and pollution |
| title | Metagenomic insights into chlorination effects on microbial antibiotic resistance in drinking water |
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