Reduced expression of MIR409‐3p in primary immune thrombocytopenia

• Published in: British journal of haematology 2013-04, Vol.161 (1), p.128-135
• Main Authors: Li, Huiyuan, Zhao, Haifeng, Xue, Feng, Zhang, Xian, Zhang, Donglei, Ge, Jing, Yang, Yanhui, Xuan, Min, Fu, Rongfeng, Yang, Renchi
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Autoimmune diseases; Autoimmune Diseases - genetics; Autoimmune Diseases - immunology; Bioinformatics; Biological and medical sciences; Case-Control Studies; Cells, Cultured; Child; DNA microarrays; Female; gamma -Interferon; Gene expression; Gene Expression Profiling - methods; Gene Expression Regulation; Hematologic and hematopoietic diseases; Humans; IFNG; Immune thrombocytopenia; Interferon-gamma - biosynthesis; Interferon-gamma - genetics; Male; Medical sciences; MicroRNAs - biosynthesis; MicroRNAs - genetics; Middle Aged; MIR409‐3p; miRNA; non-coding RNA; Peripheral blood mononuclear cells; Platelet diseases and coagulopathies; Polymerase chain reaction; Post-transcription; Proteins - genetics; Proteins - metabolism; Real-Time Polymerase Chain Reaction; Reporter gene; RNA-Binding Proteins; Thrombocytopenia; Thrombocytopenia - genetics; Thrombocytopenia - immunology; Transcription; Translation; Tumors; Young Adult
• ISSN: 0007-1048; 1365-2141
• DOI: 10.1111/bjh.12213

Summary Primary immune thrombocytopenia (ITP) is an acquired autoimmune disease with many immune dysfunctions. MicroRNAs (miRNAs) are a class of non‐coding RNAs that post‐transcriptionally regulate gene expression by messenger RNA degradation or translational repression. Accumulating evidence has demonstrated that miRNAs play a vital role in the regulation of immunological functions and prevention of autoimmunity. However, whether miRNAs are involved in the pathogenesis of ITP is still unknown. To illustrate the role of miRNAs in ITP, the expression profile of miRNAs from peripheral blood mononuclear cells (PBMCs) in ITP patients was investigated by miRNA microarray, and further validated by TaqMan real‐time polymerase chain reaction. MIR409‐3p expression was decreased in PBMCs of active ITP patients, but this recovered after effective therapy. IFNG was identified and validated as one of the targeted genes of MIR409‐3p by bioinformatic prediction and reporter gene analysis. In addition, we found DGCR8 transcript was down‐regulated in ITP patients and positively correlated with MIR409‐3p. Thus, in ITP patients, decreased DGCR8 leads to down‐regulation of MIR409‐3p, which in turn results in up‐regulation of IFNG (IFN‐γ).