Sleep Deprivation Alters Gene Expression and Antioxidant Enzyme Activity in Mice Splenocytes

Cellular defence against the formation of reactive oxygen species (ROS) involves a number of mechanisms in which antioxidant enzymes such as catalase (CAT) and superoxide dismutase (SOD) play an important role. The relation between sleep deprivation and oxidative stress has not yet been completely e...

Full description

Saved in:
Bibliographic Details
Published in:Scandinavian journal of immunology 2013-03, Vol.77 (3), p.195-199
Main Authors: Lungato, L., Marques, M. S., Pereira, V. G., Hix, S., Gazarini, M. L., Tufik, S., D'Almeida, V.
Format: Article
Language:English
Subjects:
Animals
Antioxidants - metabolism
Catalase - genetics
Catalase - metabolism
Gene Expression Regulation, Enzymologic
Lipid Peroxidation
Male
Malondialdehyde - metabolism
Mice
Oxidative Stress
Reverse Transcriptase Polymerase Chain Reaction
Sleep Deprivation - physiopathology
Spleen - cytology
Spleen - metabolism
Superoxide Dismutase - genetics
Superoxide Dismutase - metabolism
Time Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cellular defence against the formation of reactive oxygen species (ROS) involves a number of mechanisms in which antioxidant enzymes such as catalase (CAT) and superoxide dismutase (SOD) play an important role. The relation between sleep deprivation and oxidative stress has not yet been completely elucidated. Although some authors did not find evidence of this relationship, others found alterations in some oxidative stress markers in response to sleep deprivation. Thus, the objective of this study was to identify changes induced by sleep deprivation in the activity and gene expression of antioxidant enzymes in mice splenocytes, ideally corroborating a better understanding of the observed effects related to sleep deprivation, which could be triggered by oxidative imbalance. Splenocytes from mice sleep deprived for 72 h showed no significant difference in CAT and CuZnSOD gene expression compared with normal sleep mice. However, sleep‐deprived mice did show higher MnSOD gene expression than the control group. Concerning enzymatic activity, CuZnSOD and MnSOD significantly increased after sleep deprivation, despite the expression in CuZnSOD remained unchanged. Moreover, CAT activity was significantly lower after sleep deprivation. The data suggest that the antioxidant system is triggered by sleep deprivation, which in turn could influence the splenocytes homoeostasis, thus interfering in physiological responses.
ISSN:0300-9475
1365-3083
DOI:10.1111/sji.12029