Analysis of community composition of sulfur-oxidizing bacteria in hypersaline and soda lakes using soxB as a functional molecular marker

Abstract The diversity of soxB gene encoding a key enzyme of the Sox pathway sulfate thiohydrolase has been investigated in pure cultures of various halophilic and haloalkaliphilic sulfur-oxidizing bacteria (SOB) and in salt and soda lakes in southwestern Siberia and Egypt. The gene was detected in...

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Bibliographic Details
Published in:FEMS microbiology ecology 2013-05, Vol.84 (2), p.280-289
Main Authors: Tourova, Tatjana P., Slobodova, Natalija V., Bumazhkin, Boris K., Kolganova, Tatjana V., Muyzer, Gerard, Sorokin, Dimitry Y.
Format: Article
Language:English
Subjects:
Bacteria - classification
Bacteria - genetics
Bacteria - metabolism
Community composition
diversity
Ecology
Egypt
Freshwater
Gammaproteobacteria - classification
Gammaproteobacteria - genetics
Gammaproteobacteria - metabolism
Genes, Bacterial
Genetic Markers
Hydrolases - genetics
Lakes - microbiology
Microbiology
Oxidation-Reduction
phylogeny
Salinity
Salt lakes
Siberia
soda lakes
sox B
Sulfur
Sulfur - metabolism
sulfur oxidation
Thioalkalivibrio
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Summary:Abstract The diversity of soxB gene encoding a key enzyme of the Sox pathway sulfate thiohydrolase has been investigated in pure cultures of various halophilic and haloalkaliphilic sulfur-oxidizing bacteria (SOB) and in salt and soda lakes in southwestern Siberia and Egypt. The gene was detected in the majority of strains belonging to eleven SOB genera excluding members of genera Thiohalospira and Thioalkalimicrobium. The uncultured diversity of soxB in salt and soda lakes was low with a majority of detected sequences belonging to autotrophic SOB from the Gammaproteobacteria. In addition, the soxB analysis allowed detection of putative heterotrophic Gamma- and Alphaproteobacterial SOB yet unknown in culture. All clone libraries obtained from soda lakes contained soxB belonging to the genus Thioalkalivibrio in agreement with the cultivation results. Besides, representatives of the genera Halothiobacillus, Marinobacter, and Halochromatium and of the family Rhodobacteraceae have been detected in both type of saline lakes.
ISSN:0168-6496
1574-6941
DOI:10.1111/1574-6941.12056