Specific Transport of Target Molecules by Motor Proteins in Microfluidic Channels

• Published in: Chemphyschem 2013-06, Vol.14 (8), p.1618-1625
• Main Authors: Tarhan, Mehmet C., Yokokawa, Ryuji, Morin, Fabrice O., Fujita, Hiroyuki
• Format: Article
• Language: English
• Subjects: Applied fluid mechanics; Applied sciences; Biological and medical sciences; Biological Transport; Biotechnology; Biotin - chemistry; Biotin - metabolism; direct transport; Electronics; Exact sciences and technology; Fluid dynamics; Fluidics; Fundamental and applied biological sciences. Psychology; Fundamental areas of phenomenology (including applications); Kinesin - chemistry; Methods. Procedures. Technologies; Micro- and nanoelectromechanical devices (mems/nems); Microfluidic Analytical Techniques - instrumentation; microfluidics; microtubules; Microtubules - chemistry; Microtubules - metabolism; Molecular Motor Proteins - chemistry; Molecular Motor Proteins - metabolism; molecular recognition; Others; Physics; Protein Binding; Proteins; R&D; Research & development; Semiconductor electronics. Microelectronics. Optoelectronics. Solid state devices; Streptavidin - chemistry; Streptavidin - metabolism; Various methods and equipments
• ISSN: 1439-4235; 1439-7641
• DOI: 10.1002/cphc.201300022

Direct transport powered by motor proteins can alleviate the challenges presented by miniaturization of microfluidic systems. There have been several recent attempts to build motor‐protein‐driven transport systems based on simple capturing or transport mechanisms. However, to achieve a multifunctional device for practical applications, a more complex sorting/transport system should be realized. Herein, the proof of concept of a sorting device employing selective capture of distinct target molecules and transport of the sorted molecules to different predefined directions is presented. By combining the bottom‐up functionality of biological systems with the top‐down handling capabilities of micro‐electromechanical systems technology, highly selective molecular recognition and motor‐protein‐based transport is integrated in a microfluidic channel network. A good sort: The proof of concept of a sorting device employing selective capture of distinct target molecules and transport of the sorted molecules to different predefined directions is presented (see picture). It combines the bottom‐up functionality of biological systems with the top‐down handling capabilities of micro‐electromechanical systems technology.