Human β-defensin 3 inhibits antibiotic-resistant Staphylococcus biofilm formation

• Published in: The Journal of surgical research 2013-07, Vol.183 (1), p.204-213
• Main Authors: Zhu, Chen, MD, PhD, Tan, Honglue, MD, PhD, Cheng, Tao, MD, PhD, Shen, Hao, MD, PhD, Shao, Junjie, MD, PhD, Guo, Yongyuan, MD, PhD, Shi, Sifeng, MD, PhD, Zhang, Xianlong, MD, PhD
• Format: Article
• Language: English
• Subjects: Bacterial Proteins - metabolism; beta-Defensins - pharmacology; Biofilm; Biofilms - drug effects; Cell Adhesion; Gene Expression; Humans; Implant; Methicillin-resistant Staphylococcus; Methicillin-Resistant Staphylococcus aureus - drug effects; Microbial Sensitivity Tests; Microscopy, Confocal; Microscopy, Electron, Scanning; Operon; Penicillin-Binding Proteins; Phenotype; Staphylococcus epidermidis - drug effects; Surgery; Titanium; β-defensin
• ISSN: 0022-4804; 1095-8673
• DOI: 10.1016/j.jss.2012.11.048

Abstract Background Implantation-associated infections have increased significantly with the recent widespread use of medical implants. Treatments for these infections are not always successful because these infections are sometimes caused by multiantibiotic-resistant organisms. It is therefore particularly urgent to provide doctors with more effective antimicrobial agents against these antibiotic-resistant organisms. Human β-defensin 3 (hBD-3) has been shown to have strong broad-spectrum antibacterial activity. However, its effect on methicillin-resistant Staphylococcus epidermidis (MRSE) and methicillin-resistant Staphylococcus aureus (MRSA) in medical implant biofilm formation has not been reported. Methods In this study, we evaluated the effects of hBD-3 on S epidermidis ATCC 35984 (methicillin-resistant strain), MRSE287, and MRSA (ATCC43300) by evaluating bacterial adhesion, biofilm formation, and maturation. In addition, we used the spread plate method, confocal laser scanning microscopy, scanning electron microscopy, and real-time polymerase chain reaction to evaluate the effect of hBD-3. Results After evaluating biofilm adhesion and formation, we found that the number of each strain on the titanium surface was decreased in those groups exposed to 1MIC (minimum inhibitory concentration) of hBD-3 and was significantly lower than the number of colonies of the control. In the initial maturation of the biofilm, the numbers of each strain on the titanium surface from the 2MIC to 6MIC groups were significantly lower than the control. When the concentrations were further increased, hBD-3 was significantly effective against drug-resistant bacteria from the biofilms. Conclusions HBD-3 has the potential to eliminate the biofilm formation of Staphylococcus , especially antibiotic-resistant strains, effectively.