The antimicrobial effects of wood-associated polyphenols on food pathogens and spoilage organisms

• Published in: International journal of food microbiology 2013-06, Vol.164 (1), p.99-107
• Main Authors: Plumed-Ferrer, Carme, Väkeväinen, Kati, Komulainen, Heli, Rautiainen, Maarit, Smeds, Annika, Raitanen, Jan-Erik, Eklund, Patrik, Willför, Stefan, Alakomi, Hanna-Leena, Saarela, Maria, von Wright, Atte
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - pharmacology; Bacteria - drug effects; Biological and medical sciences; Caco-2 Cells; Candida tropicalis; Cell Membrane - drug effects; Food industries; Food Microbiology; Food spoilage; Fundamental and applied biological sciences. Psychology; Humans; Listeria monocytogenes; pinosylvin; Plant Extracts - pharmacology; Polyphenols - pharmacology; Saccharomyces cerevisiae; Salmonella; Staphylococcus aureus; Staphylococcus epidermidis; stilbenes; Stilbenes - pharmacology; Wood - chemistry; wood associated phenolic compounds
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/j.ijfoodmicro.2013.04.001

The antimicrobial effects of the wood-associated polyphenolic compounds pinosylvin, pinosylvin monomethyl ether, astringin, piceatannol, isorhapontin, isorhapontigenin, cycloXMe, dHIMP, ArX, and ArXOH were assessed against both Gram-negative (Salmonella) and Gram-positive bacteria (Listeria monocytogenes, Staphylococcus epidermidis, Staphylococcus aureus) and yeasts (Candida tropicalis, Saccharomyces cerevisiae). Particularly the stilbenes pinosylvin, its monomethyl ether and piceatannol demonstrated a clear antimicrobial activity, which in the case of pinosylvin was present also in food matrices like sauerkraut, gravlax and berry jam, but not in milk. The destabilization of the outer membrane of Gram-negative microorganisms, as well as interactions with the cell membrane, as indicated by the NPN uptake and LIVE/DEAD viability staining experiments, can be one of the specific mechanisms behind the antibacterial action. L. monocytogenes was particularly sensitive to pinosylvin, and this effect was also seen in L. monocytogenes internalized in intestinal Caco2 cells at non-cytotoxic pinosylvin concentrations. In general, the antimicrobial effects of pinosylvin were even more prominent than those of a related stilbene, resveratrol, well known for its various bioactivities. According to our results, pinosylvin could have potential as a natural disinfectant or biocide in some targeted applications. •Several wood-associated stilbenes were screened for antimicrobial activities against food pathogens and spoilage organisms.•Stilbenes, particularly pinosylvin and its monomethyl ether had a marked antibacterial and antifungal effect.•Pinosylvin was particularly active against Listeria monocytogenes, also when the bacterium grew intracellularly.•Pinosylvin and related compounds could have applications as targeted antimicrobials in certain food applications.