Monoclonal antibodies reacting with normal rat liver cells as probes in hepatocarcinogenesis

A series of four monoclonal antibodies was raised against suspensions of normal adult WAB/Not rat hepatocytes. An immunoperoxidase-staining technique was used to examine the distribution of determinants detected by these antibodies on frozen sections of fetal, neonatal, adult, and regenerating liver...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1984-04, Vol.44 (4), p.1611-1624
Main Authors: HOLMES, C. H, AUSTIN, E. B, FISK, A, GUNN, B, BALDWIN, R. W
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Antigen-Antibody Complex
Antigens, Neoplasm - analysis
Antigens, Surface - analysis
Biological and medical sciences
Epitopes - analysis
Gastroenterology. Liver. Pancreas. Abdomen
Immunoenzyme Techniques
Liver - immunology
Liver Neoplasms - chemically induced
Liver Neoplasms - immunology
Liver Neoplasms - pathology
Liver Neoplasms, Experimental - immunology
Liver Regeneration
Liver. Biliary tract. Portal circulation. Exocrine pancreas
Medical sciences
p-Dimethylaminoazobenzene
Rats
Rats, Inbred Strains
Tumors
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Summary:A series of four monoclonal antibodies was raised against suspensions of normal adult WAB/Not rat hepatocytes. An immunoperoxidase-staining technique was used to examine the distribution of determinants detected by these antibodies on frozen sections of fetal, neonatal, adult, and regenerating liver and on a range of 4-dimethylaminoazobenzene-induced liver lesions, including a panel of 32 primary liver carcinomas. Three of the antibodies were directed against hepatocytes, while a fourth antibody stained stromal elements within the liver. The determinants detected by the anti-hepatocyte monoclonal antibodies arose in a specific sequence during normal liver development and, when assessed in conjunction, characterized several phenotypes associated with stages in normal hepatocyte differentiation. These same antibody-defined phenotypes were expressed by the primary liver carcinomas, and the distribution of phenotypes among the tumors revealed a heterogeneity which was not evident from a conventional morphological classification. Primary liver tumors expressed a total of four antibody-defined phenotypes, whereas gamma-glutamyl transpeptidase-positive foci of hepatocytes and neoplastic nodules expressed, respectively, only one or 2 antibody-defined phenotypes. We suggest that monoclonal antibodies directed against normal liver cell components may provide a means to establish lineage relationships between cell populations involved in hepatocarcinogenesis.
ISSN:0008-5472
1538-7445