A DNA tweezer-actuated enzyme nanoreactor

The functions of regulatory enzymes are essential to modulating cellular pathways. Here we report a tweezer-like DNA nanodevice to actuate the activity of an enzyme/cofactor pair. A dehydrogenase and NAD + cofactor are attached to different arms of the DNA tweezer structure and actuation of enzymati...

Full description

Saved in:
Bibliographic Details
Published in:Nature communications 2013-07, Vol.4 (1), p.2127-2127, Article 2127
Main Authors: Liu, Minghui, Fu, Jinglin, Hejesen, Christian, Yang, Yuhe, Woodbury, Neal W., Gothelf, Kurt, Liu, Yan, Yan, Hao
Format: Article
Language:English
Subjects:
639/301/1005/1006
639/925/926
DNA - chemistry
Glucosephosphate Dehydrogenase - metabolism
Humanities and Social Sciences
multidisciplinary
NAD - metabolism
Nanoparticles - chemistry
Nucleic Acid Conformation
Optical Tweezers
Science
Science (multidisciplinary)
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The functions of regulatory enzymes are essential to modulating cellular pathways. Here we report a tweezer-like DNA nanodevice to actuate the activity of an enzyme/cofactor pair. A dehydrogenase and NAD + cofactor are attached to different arms of the DNA tweezer structure and actuation of enzymatic function is achieved by switching the tweezers between open and closed states. The enzyme/cofactor pair is spatially separated in the open state with inhibited enzyme function, whereas in the closed state, enzyme is activated by the close proximity of the two molecules. The conformational state of the DNA tweezer is controlled by the addition of specific oligonucleotides that serve as the thermodynamic driver (fuel) to trigger the change. Using this approach, several cycles of externally controlled enzyme inhibition and activation are successfully demonstrated. This principle of responsive enzyme nanodevices may be used to regulate other types of enzymes and to introduce feedback or feed-forward control loops. The control of regulatory enzymes is essential for the modulation of biochemical cellular pathways. Here, the authors fabricate a tweezer-like DNA nanodevice to actuate the activity of an enzyme/cofactor pair, and are able to control enzyme inhibition and activation over multiple cycles.
ISSN:2041-1723
2041-1723
DOI:10.1038/ncomms3127