Blue light irradiation suppresses dendritic cells activation in vitro

Blue light is a UV‐free irradiation suitable for treating chronic skin inflammation, for example, atopic dermatitis, psoriasis, and hand‐ and foot eczema. However, a better understanding of the mode of action is still missing. For this reason, we investigated whether dendritic cells (DC) are directl...

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Bibliographic Details
Published in:Experimental dermatology 2013-08, Vol.22 (8), p.558-560
Main Authors: Fischer, Michael R., Abel, Manuela, Lopez Kostka, Susanna, Rudolph, Berenice, Becker, Detlef, von Stebut, Esther
Format: Article
Language:English
Subjects:
atopic dermatitis
blue light
CD4-Positive T-Lymphocytes - cytology
CD4-Positive T-Lymphocytes - immunology
CD4-Positive T-Lymphocytes - radiation effects
Cell Separation
Coculture Techniques
Cytokines - metabolism
dendritic cells
Dendritic Cells - cytology
Dendritic Cells - immunology
Dendritic Cells - radiation effects
Humans
Immunosuppression
Inflammation
Interferon-gamma - pharmacology
Light
Lipopolysaccharides - pharmacology
Lymphocyte Activation - immunology
Lymphocyte Activation - radiation effects
Ultraviolet Rays
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Summary:Blue light is a UV‐free irradiation suitable for treating chronic skin inflammation, for example, atopic dermatitis, psoriasis, and hand‐ and foot eczema. However, a better understanding of the mode of action is still missing. For this reason, we investigated whether dendritic cells (DC) are directly affected by blue light irradiation in vitro. Here, we report that irradiation neither induced apoptosis nor maturation of monocyte‐derived and myeloid DC. However, subsequent DC maturation upon LPS/IFNγ stimulation was impaired in a dose‐dependent manner as assessed by maturation markers and cytokine release. Moreover, the potential of this DC to induce cytokine secretion from allogeneic CD4 T cells was reduced. In conclusion, unlike UV irradiation, blue light irradiation at high and low doses only resulted in impaired DC maturation upon activation and a reduced subsequent stimulatory capacity in allogeneic MLRs with strongest effects at higher doses.
ISSN:0906-6705
1600-0625
DOI:10.1111/exd.12193