Probe-based confocal laser endomicroscopy of the respiratory tract: A data consistency analysis

Summary Background Probe-based confocal laser endomicroscopy is a novel imaging tool in the field of respiratory medicine. It enables a real-time and bedside qualitative characterization at the level of small airways but due to a relatively small field of view the reliability of quantification remai...

Full description

Saved in:
Bibliographic Details
Published in:Respiratory medicine 2013-08, Vol.107 (8), p.1234-1240
Main Authors: Yserbyt, J, Dooms, C, Decramer, M, Verleden, G.M
Format: Article
Language:English
Subjects:
Agreements
Bronchoscopy
Bronchoscopy - methods
Bronchoscopy - standards
Confocal laser endomicroscopy
Humans
Lasers
Lung Transplantation
Microscopy, Confocal - methods
Microscopy, Confocal - standards
Point-of-Care Systems
Postoperative Care - methods
Postoperative Care - standards
Pulmonary Alveoli - anatomy & histology
Pulmonary/Respiratory
Repeatability testing
Statistical analysis
Studies
Test–retest reliability
Transplants & implants
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Summary Background Probe-based confocal laser endomicroscopy is a novel imaging tool in the field of respiratory medicine. It enables a real-time and bedside qualitative characterization at the level of small airways but due to a relatively small field of view the reliability of quantification remains uncertain. Methods Twenty-six lung transplant recipients were subjected to two consecutive alveoloscopic imaging procedures within a median time interval of 90 days to analyze test–retest reliability. Only patients in a stable clinical condition were analyzed. We studied alveolar duct diameter, elastic fiber thickness at the alveolar level, the number of autofluorescent cells at the level of the alveolar space, the diameter of autofluorescent alveolar cells and their autofluorescence intensity. Results Intraclass correlation coefficients ranged from 0.56 for elastic fiber thickness, 0.62 for alveolar duct diameter, 0.29 for alveolar cell diameter, 0.74 for cellularity to 0.78 for alveolar cell autofluorescence. Conclusion Probe-based confocal laser endomicroscopy enables imaging and quantitative measurements at the level of small airways and alveolar ducts. Test–retest reliability is good for cellularity and autofluorescence quantification but only moderate for morphometric analysis of elastic fibers and alveolar ducts.
ISSN:0954-6111
1532-3064
DOI:10.1016/j.rmed.2013.04.018