Flavipin in Chaetomium globosum CDW7, an endophytic fungus from Ginkgo biloba, contributes to antioxidant activity

• Published in: Applied microbiology and biotechnology 2013-08, Vol.97 (16), p.7131-7139
• Main Authors: Ye, Yonghao, Xiao, Yu, Ma, Liang, Li, Hongxia, Xie, Zhenglu, Wang, Minghua, Ma, Haitian, Tang, Huaiwu, Liu, Junyan
• Format: Article
• Language: English
• Subjects: Acids; Analysis; Antioxidants; Antioxidants - metabolism; Biomedical and Life Sciences; Biotechnological Products and Process Engineering; Biotechnology; Chaetomium - classification; Chaetomium - isolation & purification; Chaetomium - metabolism; Chaetomium globosum; Cocoa; Correlation coefficient; Crop diseases; DNA, Fungal - chemistry; DNA, Fungal - genetics; DNA, Ribosomal Spacer - chemistry; DNA, Ribosomal Spacer - genetics; Endophytes; Endophytes - classification; Endophytes - isolation & purification; Endophytes - metabolism; Fermentation; Flowers & plants; Free Radical Scavengers - metabolism; Fungi; Ginkgo biloba; Ginkgo biloba - microbiology; Laboratories; Life Sciences; Metabolites; Microbial Genetics and Genomics; Microbiology; Molecular Sequence Data; Nematoda; o-Phthalaldehyde - analogs & derivatives; o-Phthalaldehyde - metabolism; Proteins; Sequence Analysis, DNA; Studies
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-013-5013-8

1,2-Benzenedicarboxaldehyde-3,4,5-trihydroxy-6-methyl (flavipin) was found to be antagonistic against nematodes and fungi. Here we demonstrated that flavipin is a potent antioxidant in vitro and in vivo, which has great potential in the therapy for free radical-associated diseases. Therefore, flavipin-producing bio-source was screened from 80 endophytes in Ginkgo biloba . Seven endophytic fungi were able to synthesize antioxidant substances and identified by ITS rDNA sequences. Among them, Chaetomium globosum CDW7 was a remarkable producer of flavipin. The fermentation parameters of CDW7 were then optimized for high flavipin production. Cultured under the optimal condition (25 °C, 100/250 mL flask, 12 discs/flask, 150 rpm, pH 6.5) for 14 days, CDW7 was able to synthesize flavipin at a production of 315.5 mg/L. In addition, flavipin output was positively correlated to antioxidant activities of crude extracts with a correlation coefficient of 0.8235, indicating that flavipin was the major antioxidant component of CDW7's metabolites. These data demonstrated that CDW7 was a highly yielded bio-source of antioxidant flavipin.