Indirect Chemiluminescence-based Determination of Catecholamines in Pharmaceutical Formulations by Furandicarboxylate Derivative as a Novel Blue Fluorescer in Peroxyoxalate-H2O2 System

• Published in: Analytical Sciences 2013/08/10, Vol.29(8), pp.815-821
• Main Authors: CHAICHI, Mohammad Javad, KHAJVAND, Tahereh, MEHRZAD, Jalil, ASGHARI, Sakineh, QANDALEE, Mohammad
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; catecholamine pharmaceuticals; Catecholamines - analysis; Chemistry; Dicarboxylic Acids - chemistry; furandicarboxylate derivative; Furans - chemistry; H2O2; Hydrogen Peroxide - chemistry; kinetic parameters; Limit of Detection; Luminescence; Oxalates - chemistry; Peroxyoxalate chemiluminescence; Pharmaceutical Preparations - chemistry
• ISSN: 0910-6340; 1348-2246
• DOI: 10.2116/analsci.29.815

A novel, simple, cheap, and high sensitivity batch chemiluminescent method for the determination of catecholamine drugs, epinephrine (E), dopamine (DA) and methyldopa (MD) at microgram levels in pharmaceutical formulations is described. The method is based on a chemiluminescence (CL) system arising from the reaction of bis(2,4,6-trichlorophenyl) oxalate (TCPO) with H2O2 in the presence of a novel fluorescer, furandicarboxylate, and is proposed as a new analytical method for the determination of catecholamines. The method is based on the inhibition of CL emission by DA and its enhancement by E and MD. Under optimal conditions, good linear ranges were obtained, 0.5 – 12.7, 0.06 – 1.83 and 0.069 – 3.52 μg/mL with detection limits of 0.30, 0.03 and 0.04 μg/mL (S/N = 3) for DA, E and MD, respectively. Moreover, a pooled-intermediate model was used to determine the kinetic parameters of CL with and without catecholamines and a possible CL mechanism was discussed.