Attaching DNA to Nanoceria: Regulating Oxidase Activity and Fluorescence Quenching

Cerium oxide nanoparticles (nanoceria) have recently emerged as a nanozyme with oxidase activity. In this work, we present a few important interfacial properties of nanoceria. First, the surface charge of nanoceria can be controlled not only by adjusting pH but also by adsorption of simple inorganic...

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Bibliographic Details
Published in:ACS applied materials & interfaces 2013-08, Vol.5 (15), p.6820-6825
Main Authors: Pautler, Rachel, Kelly, Erin Y, Huang, Po-Jung Jimmy, Cao, Jing, Liu, Biwu, Liu, Juewen
Format: Article
Language:English
Subjects:
Adsorption
Animals
Anions
Biosensing Techniques
Catalysis
Cerium - chemistry
Citric Acid - chemistry
DNA - chemistry
Humans
Hydrogen-Ion Concentration
Nanoparticles - chemistry
Oxidoreductases - chemistry
Phosphates - chemistry
Spectrometry, Fluorescence - methods
Static Electricity
Surface Properties
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Summary:Cerium oxide nanoparticles (nanoceria) have recently emerged as a nanozyme with oxidase activity. In this work, we present a few important interfacial properties of nanoceria. First, the surface charge of nanoceria can be controlled not only by adjusting pH but also by adsorption of simple inorganic anions. Adsorption of phosphate and citrate gives negatively charged surface over a broad pH range. Second, nanoceria adsorbs DNA via the DNA phosphate backbone in a sequence-independent manner; DNA adsorption inhibits its oxidase activity. Other anionic polymers display much weaker inhibition effects. Adsorption of simple inorganic phosphate does not have the inhibition effect. Third, nanoceria is a quencher for many fluorophores. These discoveries provide an important understanding for further use of nanoceria in biosensor development, materials science, and nanotechnology.
ISSN:1944-8244
1944-8252
DOI:10.1021/am4018863