Zinc toxicity in ectomycorrhizal Pinus sylvestris

• Published in: Plant and soil 1992-06, Vol.143 (2), p.201-211
• Main Authors: Colpaert, J. van (Katholieke Univ. Leuven (Belgium). Lab. of Plant Ecology), Assche, J.A. van
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; CINC; Deficiencies. Phytotoxicity of elements. Salinity; FITOTOXICIDAD; Fundamental and applied biological sciences. Psychology; Fungi; General agronomy. Plant production; Host plants; IMMOBILISATION; IMMOBILIZATION; INMOVILIZACION; Mycelium; MYCORHIZE; MYCORRHIZAE; Mycorrhizas; PHYTOTOXICITE; PHYTOTOXICITY; PINUS SYLVESTRIS; Plant growth; Plant roots; Plants; PLANTULAS; PLANTULE; SEEDLINGS; Soil-plant relationships. Soil fertility. Fertilization. Amendments; THELEPHORA; Toxicity; ZINC
• ISSN: 0032-079X; 1573-5036
• DOI: 10.1007/BF00007874

Six strains of ectomycorrhizal fungi were compared for their ability to increase zinc tolerance in Pinus sylvestris L. seedlings. Pioneer and 'late-stage' fungi as well as one strain collected from a Zn-polluted site were included in the study. The accumulation of zinc in the host plants was determined at two different sublethal substrate Zn concentrations. The mycobionts varied considerably in their protection of the autobionts against zinc toxicity. Several fungal species reduced zinc accumulation in the pine seedlings. A Thelephora terrestris (Ehrh.) Fr. Strain, however, increased the Zn concentration in its host plants. Specific zinc-retaining capacity of the mycelium and density of the extramatrical mycelium were important features determining the effectiveness of the zinc retention in the fungal symbiont. The experiments were carried out in a growth chamber where plants were cultivated in root observation chambers. The determination of the extent of substrate colonization showed that an elevated zinc concentration in a substrate might be an important stress factor for the growth of an ectomycorrhizal fungus. The growth of the extramatrical mycelium of some species was inhibited at the highest zinc concentration supplied. A determination of the in vitro zinc tolerance of the fungi could not always predict their tolerance as symbionts, where the latter characteristic did not determine the Zn retention capacity of their mycelium. In this paper we also discuss the possibility that on Zn-polluted soils ectomycorrhizal species and strains are selected that are Zn-tolerant and, in addition, are able to protect their own energy source, the autobiont, from toxicity. These mycorrhizal fungi act as a safety net, that can immobilize large amounts of zinc, thus preventing transport to the host plant.