Use of real-time PCR technique in determination of major fibrolytic and non fibrolytic bacteria present in Indian Surti buffaloes (Bubalus bubalis)

In the milk industry in India, buffalo breeds are most commonly used for milk production. Efficiency of fiber digestion in ruminants is critical for animal productivity. Bacteria play an important role in fiber digestion and utilization. Absolute quantification real-time PCR was used to quantify ten...

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Bibliographic Details
Published in:Polish journal of microbiology 2013, Vol.62 (2), p.195-200
Main Authors: Singh, Krishna M, Tripathi, Ajai K, Pandya, Paresh R, Parnerkar, Subhash, Rank, Dharamshibhai N, Kothari, Ramesh K, Joshi, Chaitanya G
Format: Article
Language:English
Subjects:
Animals
Body Fluids - microbiology
Buffaloes - microbiology
Diet - veterinary
Dietary Fiber - metabolism
DNA, Bacterial - genetics
India
Real-Time Polymerase Chain Reaction - veterinary
RNA, Bacterial - genetics
RNA, Ribosomal, 16S - genetics
Rumen - microbiology
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Summary:In the milk industry in India, buffalo breeds are most commonly used for milk production. Efficiency of fiber digestion in ruminants is critical for animal productivity. Bacteria play an important role in fiber digestion and utilization. Absolute quantification real-time PCR was used to quantify ten bacterial species in rumen fluid of Surti buffalo fed green fodder, dry roughage and compound concentrate mixture. Abundance of each target taxon was calculated as a fraction of the total 16S rRNA gene copies in the samples, using taxon-specific primers. Bacterial populations showed a clear predominance of Ruminococcus albus, which comprised 5.66% of the bacterial rRNA gene copies in the samples. However, only 0.9% to 4.24% of the bacterial rRNA gene copies were represented by the ruminal Fibrobacter succinogenes, Ruminococcus flavefaciens and Prevotella species. The proportion of rRNA gene copies attributable to Selenomonas ruminantium, Streptococcus bovis, Ruminobacter amylophilus, Treponema bryantii and Anaerovibrio lipolytica was even less abundant, each comprising < 0.11% of the bacterial rRNA gene copies. The data suggest that the aggregate abundance of the most intensively studied ruminal bacterial species is relatively low and that a large fraction of the uncultured population represents a single bacterial genus.
ISSN:1733-1331
2544-4646
DOI:10.33073/pjm-2013-025