Immunogenicity and safety of H1N1 influenza hemagglutinin protein expressed in a baculovirus system

ABSTRACT Although most influenza vaccines are produced in eggs, new types of vaccines must be developed. In this study, the immunogenicity and safety of a baculovirus‐expressed hemagglutinin (HA) of H1N1 influenza virus (Korea/01/2009; designated “HA‐Bac‐K”) was compared with those of a commercially...

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Bibliographic Details
Published in:Microbiology and immunology 2013-09, Vol.57 (9), p.660-664
Main Authors: Na, Ha-Na, Kim, Kyung Hyun, Song, Man Ki, Park, Hye-lim, Lee, Eun-young, Shim, Seung-Hyun, Park, Sooho, Nam, Jae-Hwan
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - immunology
Baculoviridae - genetics
Baculoviridae - metabolism
Female
Ferrets
Gene Expression
hemagglutinin
Hemagglutinin Glycoproteins, Influenza Virus - adverse effects
Hemagglutinin Glycoproteins, Influenza Virus - genetics
Hemagglutinin Glycoproteins, Influenza Virus - immunology
Humans
Influenza A Virus, H1N1 Subtype - genetics
Influenza A Virus, H1N1 Subtype - immunology
Influenza Vaccines - administration & dosage
Influenza Vaccines - adverse effects
Influenza Vaccines - genetics
Influenza Vaccines - immunology
influenza virus
Influenza, Human - immunology
Influenza, Human - prevention & control
Influenza, Human - virology
Mice
Swine flu
vaccine
Vaccines
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Summary:ABSTRACT Although most influenza vaccines are produced in eggs, new types of vaccines must be developed. In this study, the immunogenicity and safety of a baculovirus‐expressed hemagglutinin (HA) of H1N1 influenza virus (Korea/01/2009; designated “HA‐Bac‐K”) was compared with those of a commercially available baculovirus‐expressed HA (designated “HA‐Bac‐C”) and an Escherichia coli‐expressed HA (designated “HA‐E. Coli‐K”). HA‐Bac‐K succeeded in inducing hemagglutination inhibition and neutralization antibodies in mouse and ferret models. The different immunogenicities observed may be attributable to the different expression systems and purification protocols used. Our work suggests that HA expressed in a baculovirus system is an effective and safe candidate influenza vaccine.
ISSN:0385-5600
1348-0421
DOI:10.1111/1348-0421.12079