Development of a novel codominant molecular marker for chili veinal mottle virus resistance in Capsicum annuum L

• Published in: Euphytica 2013-09, Vol.193 (2), p.197-205
• Main Authors: Lee, Heung-Ryul, An, Hye Jung, You, Young Gon, Lee, Jangha, Kim, Hyoun-Joung, Kang, Byoung-Cheorl, Harn, Chee Hark
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Amplified fragment length polymorphism; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Capsicum annuum; Fundamental and applied biological sciences. Psychology; Genes; Genetics and breeding of economic plants; Health aspects; Life Sciences; Pest resistance; Plant Genetics and Genomics; Plant pathogens; Plant Pathology; Plant Physiology; Plant Sciences; Varietal selection. Specialized plant breeding, plant breeding aims
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1007/s10681-013-0897-z

Chili veinal mottle virus (ChiVMV) is one of the most destructive pepper pathogens in Asia. Development of ChiVMV-resistant cultivars is necessary to control ChiVMV infection on pepper farms. However, sources of variation for ChiVMV resistance have not been identified and only a recessive resistance gene has been identified. We initially screened 30 pepper lines from several countries using inoculation tests to further examine inheritance of ChiVMV resistance, to establish a relevant breeding program, and to develop a new resistant line. Here, we report a new genetically dominant source of resistance to ChiVMV in pepper. Secondly, we found two amplified fragment length polymorphisms linked to dominant resistance and converted them into high-resolution melting markers, which were located on chromosome 6. Furthermore, we obtained a cleaved amplified polymorphic sequence marker that was closer to the ChiVMV resistance locus using comparative mapping. The newly discovered marker, related to a single dominant gene, will help researchers develop a new ChiVMV-resistant pepper cultivar.