Distribution of bacterial endophytes in peanut seeds obtained from axenic and control plant material under field conditions

• Published in: Plant and soil 2013-10, Vol.371 (1/2), p.367-376
• Main Authors: Sobolev, V. S., Orner, V. A., Arias, R. S.
• Format: Article
• Language: English
• Subjects: Agricultural soils; Agrology; Agronomy. Soil science and plant productions; Animal, plant and microbial ecology; Arachis hypogaea; Aspergillus flavus; Bacillus; Bacillus thuringiensis; Bacteria; Biological and medical sciences; Biomedical and Life Sciences; Deoxyribonucleic acid; Diseases and pests; DNA; Ecology; Endophytes; Endosymbionts; Fundamental and applied biological sciences. Psychology; General agronomy. Plant production; Health aspects; Host-parasite relationships; Legumes; Life Sciences; Peanuts; Physiological aspects; Plant Physiology; Plant Sciences; Plants; Regular Article; Seeds; Soil analysis; Soil bacteria; Soil microorganisms; Soil Science & Conservation; Soil-plant relationships. Soil fertility; Soil-plant relationships. Soil fertility. Fertilization. Amendments
• ISSN: 0032-079X; 1573-5036
• DOI: 10.1007/s11104-013-1692-2

Background and Aims The role and linkage of endophytic bacteria to resistance of peanut seeds to biotic stress is poorly understood. The aims of the present study were to survey the experimental (axenic) and control (conventional) peanut plants for the predominant endophytic bacteria, and to characterize isolates with activity against selected A. flavus strains. Methods Young axenic plants were grown from presumably bacteria-free embryos in the lab, and then they were grown in a field. Endophytic bacterial species were identified by the analysis of DNA sequences of their 16S-ribosomal RNA gene. DNA extracted from soil was also analyzed for predominant bacteria. Results Mature seeds from the experimental and control plants contained several species of nonpathogenic endophytic bacteria. Among the eight bacterial species isolated from seeds, and DNA sequences detected in soil, Bacillus thuringiensis was dominant. All B. amyloliquefaciens isolates, the second abundant species in seeds demonstrated activity against A. flavus. This effect was not observed with any other bacterial isolates. There was no significant difference in number and relative occurrence of the two major bacterial species between the experimental and conventionally grown control seeds. Conclusion Endophytic bacterial colonization derives from local soil and not from the seed source, and the peanut plant accommodates only selected species of bacteria from diverse soil populations. Some bacterial isolates showed antibiosis against A. flavus.