Adult Opisthorchis felineus major protein fractions deduced from transcripts: Comparison with liver flukes Opisthorchis viverrini and Clonorchis sinensis

• Published in: Experimental parasitology 2013-10, Vol.135 (2), p.297-306
• Main Authors: Pomaznoy, Mikhail, Tatkov, Sergey, Katokhin, Alexey, Afonnikov, Dmitry, Babenko, Vladimir, Furman, Dagmara, Brusentsov, Ilya, Belavin, Pavel, Najakshin, Alexandr, Guselnikov, Sergey, Vasiliev, Gennady, Sivkov, Anton, Prokhortchouk, Egor, Skryabin, Konstantin, Mordvinov, Viatcheslav
• Format: Article
• Language: English
• Subjects: adults; Amino Acid Sequence; amino acid sequences; Animals; biologists; cathepsin F; Clonorchis sinensis; Clonorchis sinensis - chemistry; Clonorchis sinensis - classification; Clonorchis sinensis - genetics; Contig Mapping; Cricetinae; Cyprinidae - parasitology; Cysteine Proteases - chemistry; Cysteine Proteases - genetics; Egg Proteins - chemistry; Egg Proteins - genetics; EST; expressed sequence tags; Expressed Sequence Tags - chemistry; Fasciola; Fish Diseases - parasitology; genes; glutathione transferase; Glutathione Transferase - chemistry; Glutathione Transferase - genetics; health care workers; Helminth Proteins - chemistry; Helminth Proteins - genetics; liver flukes; Mesocricetus; molecular biology; Molecular Sequence Annotation; Molecular Sequence Data; myoglobin; Myoglobin - chemistry; Myoglobin - genetics; Opisthorchiasis - parasitology; Opisthorchiasis - veterinary; Opisthorchis - chemistry; Opisthorchis - classification; Opisthorchis - genetics; Opisthorchis felineus; Opisthorchis viverrini; parasitology; Phylogeny; Protein Precursors - chemistry; Protein Precursors - genetics; ribosomal proteins; Ribosomal Proteins - chemistry; Ribosomal Proteins - genetics; RNA, Messenger - genetics; RNA, Messenger - isolation & purification; Schistosoma; transcriptome; Transcriptome - genetics; Transcriptomics
• ISSN: 0014-4894; 1090-2449
• DOI: 10.1016/j.exppara.2013.07.011

•We obtained the first transcriptomic data for Opisthorchis felineus.•Sequences were annotated with BLAST and Interproscan algorithms.•Trematode specific orthologous clusters were obtained.•Phylogenetic analysis of Opisthorchiidae species was undertaken. The epidemiologically important liver flukes Opisthorchis felineus, Opisthorchis viverrini, and Clonorchis sinensis are of interest to health professionals, epidemiologists, pharmacologists, and molecular biologists. Recently the transcriptomes of the latter two species were intensively investigated. However our knowledge on molecular biology of O. felineus is scarce. We report the first results of the O. felineus transcriptome analysis. We isolated and annotated a total of 2560 expressed sequence tag (EST) sequences from adult O. felineus (deposited within the database of expressed sequence tags (dbEST), under accession numbers GenBank: JK624271–JK626790, JK006511–JK006547, JK649790–JK649792). Clustering and analysis resulted in the detection of 267 contigs. Of the protein sequences deduced from these, 82% had homologs in the NCBI (nr) protein database and 63% contained conserved domains, allowing the functions to be interpreted using the Gene Ontology terms. Comprehensive analysis of Opisthorchiidae- and Trematoda-specific substitutions within amino acid sequences deduced for the proteins myoglobin, vitelline precursor protein, cathepsin F, and 28kDa glutathione transferase was carried out. The gene set of the 32 ribosomal proteins for the three Opisthorchiidae species with the addition of available Schistosoma and Fasciola orthologs was created and is provided in the supplementary. The orthologous gene set created was used for inferring phylogeny within the Trematoda with special attention to interrelations within the Opisthorchiidae. The phylogenetic analysis revealed a closer relationship between C. sinensis and O. viverrini and some divergence of O. felineus from either O. viverrini or C. sinensis.