Inhibition of photosynthesis by chilling in moderate light: a comparison of plants sensitive and insensitive to chilling

• Published in: Planta 1989-12, Vol.178 (4), p.545-552
• Main Authors: Hodgson, Richard A.J., Raison, John K.
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Chloroplasts; Cooling; Cucumbers; Fundamental and applied biological sciences. Psychology; Leaves; Lipids; Metabolism; Photoinhibition; Photosynthesis; Photosynthesis, respiration. Anabolism, catabolism; Plant physiology and development; Plants; Spinach; Thylakoids
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/BF00963825

Photosynthetic activity, in leaf slices and isolated thylakoids, was examined at 25° C after preincubation of the slices at either 25° C or 4° C at a moderate photon flux density (PFD) of 450 μmol·m-2·s-1, or at 4° C in the dark. The plants used were Spinacia oleracea L., Cucumis sativus L. and Nerium oleander L. which was acclimated to growth at 20° C or 45° C. The were grown at a PFD of 550 μmol·m-2·s-1. Photosynthesis, measured as CO2-dependent O2 evolution, was not inhibited in leaf slices from any plant after preincubation at 25° C at a moderate PFD or at 4° C in the dark. However, exposure to 4° C at a moderate PFD induced an inhibition of CO2-dependent O2 evolution within 1 h in C. sativus, a chilling-sensitive plant, and in 45° C-grown N. oleander. The inhibition in these plants after 5 h reached 80% and 40%, respectively, and was independent of the CO2 concentration but was reduced at O2 concentrations of less than 3%. Methyl-viologen-dependent O2 exchange in leaf slices from these plants was not inhibited. There was no photoxidation of chlorophyll, in isolated thylakoids, or any inhibition of electron transport at photosystem (PS)II, PSI or through both photosystems which would account for the inhibition of photosynthesis. The conditions which inhibit photosynthesis in chilling-sensitive plants do not cause inhibition in S. oleracea, a chilling-insensitive plant, or in 20° C-grown N. oleander. The CO2-dependent photosynthesis, measured at 5° C, was reduced to about 3% of that recorded at 25° C in chilling-sensitive plants but only to about 30% in the chilling-insensitive plants. Methyl-viologen-dependent O2 exchange, measured at 5° C, was greater than 25% of the activity at 25° C in all the plants. The results indicate that the mechanism of the chilling-induced inhibition of photosynthesis does not involve damage to PSII. That inhibition of photosynthesis is observed only in the chilling-sensitive plants indicates it is related, in some way, to the disproportionate decrease in photosynthetic activity in these plants at chilling temperatures.