Gene expression in the soybean seed axis during germination and early seedling growth

Copy-DNA clones have been obtained that distinguish eight messenger mRNAs, moderately abundant in the axes of the germinating soybean (Glycine max (L.) Merr.) seedling. These clones have been used to characterize the size of the mRNAs and to analyze the accumulation of the mRNAs at different time po...

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Bibliographic Details
Published in:Planta 1987-02, Vol.170 (2), p.209-216
Main Authors: Datta, K. (Fox Chase Cancer Center, Philadelphia, Pennsylvania (USA). Inst. for Cancer Research), Parker, H, Averyhart-Fullard, V, Schmidt, A, Marcus, A
Format: Article
Language:English
Subjects:
ARN
Axes
Biological and medical sciences
Embryos
Entwicklungsstadium
Fundamental and applied biological sciences. Psychology
Genauspraegung
Gene expression
GERMINACION
GERMINATION
Glycine
GLYCINE MAX
GRAINE
Messenger RNA
Molecular and cellular biology
Molecular genetics
mRNS
Nukleinsaeure
PLANTULAS
PLANTULE
Polyribosomes
RNA
Saemlingswachstum
Samenkeimung
Seedling growth
SEEDLINGS
SEEDS
SEMILLA
Soybeans
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Summary:Copy-DNA clones have been obtained that distinguish eight messenger mRNAs, moderately abundant in the axes of the germinating soybean (Glycine max (L.) Merr.) seedling. These clones have been used to characterize the size of the mRNAs and to analyze the accumulation of the mRNAs at different time points and in different parts of the axis during germination and early seedling growth. Three of the mRNAs accumulate to a substantial level by 9 h, a time point before either the beginning of growth or the accumulation of polyribosomes. Four other mRNAs reach a substantial level only at 24 h, a period when rapid seedling growth is occurring. Those mRNAs whose accumulation begins at 24 h were found only in the top (hypocotyl) half of the 24-h seedlings, while the remaining mRNAs were present also in the bottom half of the seedlings in different amounts. By 44 h, the bottom 0.5 cm of the seedlings, i.e., the region of meristematic growth, had little or none of the mRNAs, with the exception of one mRNA. These temporal and spatial observations indicate that many of the mRNAs are not involved simply in the general maintenance of ongoing cell proliferation, but that they may be related to differentiation during early seedling formation. Further, the early accumulating mRNAs may be functioning in regulating the onset of seedling growth.
ISSN:0032-0935
1432-2048
DOI:10.1007/BF00397890