Stable transformation of papaya via microprojectile bombardment

Stable transformation of papaya (Carica papaya L.) has been achieved following DNA delivery via high velocity microprojectiles. Three types of embryogenic tissues, including immature zygotic embryos, freshly explanted hypocotyl sections, and somatic embryos derived from both, were bombarded with tun...

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Bibliographic Details
Published in:Plant cell reports 1990-08, Vol.9 (4), p.189-194
Main Authors: Fitch, M.M.M, Manshardt, R.M, Gonsalves, D, Slightom, J.L, Sanford, J.C
Format: Article
Language:English
Subjects:
Carica papaya
chromosome mapping
culture media
DNA
embryo (plant)
gene expression
genetic transformation
injection
regeneration
somatic embryogenesis
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Summary:Stable transformation of papaya (Carica papaya L.) has been achieved following DNA delivery via high velocity microprojectiles. Three types of embryogenic tissues, including immature zygotic embryos, freshly explanted hypocotyl sections, and somatic embryos derived from both, were bombarded with tungsten particles carrying chimeric NPTII and GUS genes. All tissue types were cultured prior to and following bombardment on half-strength MS medium supplemented with 10 mg 1(-1) 2,4-D, 400 mg 1(-1) glutamine, and 6% sucrose. Upon transfer to 2,4-D-free medium containing 150 mg 1(-1) kanamycin sulfate, ten putative transgenic isolates produced somatic embryos and five regenerated leafy shoots. Leafy shoots were produced six to nine months following bombardment. Tissues from 13 of these isolates were assayed for NPTII activity, and 10 were positive. Six out of 15 isolates assayed for GUS expression were positive. Three isolates were positive for both NPTII and GUS.
ISSN:0721-7714
1432-203X
DOI:10.1007/bf00232177