Effect of several environmental parameters on carbon metabolism in Histosols

• Published in: Microbial ecology 1980-12, Vol.5 (4), p.329-336
• Main Author: Tate, R.L. III
• Format: Article
• Language: English
• Subjects: Acid soils; Agricultural soils; Agrology; Organic soils; Oxidation; Sedimentary soils; soil chemistry; Soil microorganisms; Soil organic matter; soil physics; Soil samples; soil science; Soil water
• ISSN: 0095-3628; 1432-184X
• DOI: 10.1007/BF02020340

High specific activity14C-labeled glucose, succinate, acetate, salicylate, and amino acids were used to examine carbon metabolism by the microbial community of Pahokee muck (a Lithic medisaprist), a drained, cultivated soil of the Florida Everglades. Variations in carbon oxidation were observed from the end of the wet season through the dry season in a fallow (bare) field. Evolution of14CO2varied with the substrate added and time. Calculation of14CO2evolution for each substrate as a proportion of total respiration of the microbial community which was measured by succinate oxidation (relative oxidation) allowed for determination of the proportion of metabolic activity contributed by the oxidation of each carbon source. Except for the May sample when an approximate 30% decline in relative salicylate oxidation activity was observed, the proportion of total catabolic activity contributed by salicylate oxidation and acetate degradation was constant with time. Relative oxidation of glucose and amino acids ranged from 0.12 to 0.52 and 0.10 to 0.23, respectively. At two times during the dry season, the effect of depth of soil and crop on the carbon oxidation was examined. Relative acetate and amino acid oxidation were constant with depth whereas statistically significant variation was observed in glucose and salicylate oxidation. Generally, with the latter substrates, the activity declined with increased soil depth. Greatest effect of crop on these metabolic activities was noted with oxidation of salicylate in soils from a St. Augustinegrass [Stenatophrum secundatum (Walt.) Kuntz] pasture. In these soils, oxidation of salicylate was nearly double that of the fallow field or of soil planted with sugarcane (Saccharum sp.).