In vitro study of transgenic tobacco expressing Arabidopsis wild type and mutant acetohydroxyacid synthase genes

Genes coding for the enzyme acetohydroxyacid synthase, often referred to as acetolactate synthase (AHAS, ALS; EC 4.1.3.18), from wild type Arabidopsis thaliana and a sulfonylurea-resistant mutant line GH50 (csrl-1; Haughn et al. 1988) were introduced in Nicotiana tabacum. Both genes were expressed a...

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Bibliographic Details
Published in:Plant cell reports 1990-04, Vol.8 (11), p.643-646
Main Authors: Charest, P.J, Hattori, J, DeMoor, J, Iyer, V.N, Miki, B.L
Format: Article
Language:English
Subjects:
Arabidopsis thaliana
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
gene expression
genes
genetic code
Genetic engineering
Genetic technics
genetic transformation
herbicides
ligases
markers
Methods. Procedures. Technologies
mutants
Nicotiana tabacum
resistance
selection
Transgenic animals and transgenic plants
Transgenic plants
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Summary:Genes coding for the enzyme acetohydroxyacid synthase, often referred to as acetolactate synthase (AHAS, ALS; EC 4.1.3.18), from wild type Arabidopsis thaliana and a sulfonylurea-resistant mutant line GH50 (csrl-1; Haughn et al. 1988) were introduced in Nicotiana tabacum. Both genes were expressed at high levels with the 35S promoter. The csrl-1 gene conferred high levels of resistance to chlorsulfuron whereas the wild type gene did not. As selectable markers, chimaeric AHAS genes yielded transgenic plants on chlorsulfuron but at much lower efficiencies than with a chimaeric neomycin phosphotransferase gene on kanamycin (Sanders et al. 1987). Shoot differentiation from leaf discs was delayed on chlorsulfuron by 4-6 weeks. This study indicated a role for mutant AHAS genes in the genetic manipulation of herbicide resistance in transgenic plants but as selectable markers for plant cells undergoing differentiation no advantage over other genes was perceived.
ISSN:0721-7714
1432-203X
DOI:10.1007/bf00269983