Control of human vascular tone by prostanoids derived from perivascular adipose tissue

• Published in: Prostaglandins & other lipid mediators 2013-12, Vol.107, p.13-17
• Main Authors: Ozen, Gulsev, Topal, Gokce, Gomez, Ingrid, Ghorreshi, Arézou, Boukais, Kamel, Benyahia, Chabha, Kanyinda, Larry, Longrois, Dan, Teskin, Onder, Uydes-Dogan, B. Sonmez, Norel, Xavier
• Format: Article
• Language: English
• Subjects: Adipose Tissue, White - metabolism; Aged; Dinoprostone - pharmacology; Dinoprostone - physiology; Epoprostenol - pharmacology; Epoprostenol - physiology; Female; Human; Humans; Indomethacin - pharmacology; Inhibitory Concentration 50; Internal mammary artery; Male; Mammary Arteries - drug effects; Mammary Arteries - physiology; Middle Aged; Muscle Contraction; Muscle, Smooth, Vascular - drug effects; Muscle, Smooth, Vascular - physiology; Norepinephrine - pharmacology; Perivascular adipose tissue; Prostanoids; Saphenous vein; Saphenous Vein - drug effects; Saphenous Vein - physiology; Tissue Culture Techniques; Vasoconstriction; Vasoconstrictor Agents - pharmacology
• ISSN: 1098-8823
• DOI: 10.1016/j.prostaglandins.2013.06.002

•PVAT of SV and IMA attenuated NA-induced contraction.•PVAT surrounding SV released PGE2 and PGI2.•The involvement of PGE2 and PGI2 release from PVAT in the control of vascular tone in SV was shown.•PVAT of IMA exerted its anti-contractile effect independently from prostanoids.•Retaining PVAT in SV and IMA might have clinical implications to improve bypass graft patency. Perivascular adipose tissue (PVAT) surrounds most vessels and has now been recognized as a regulator of vascular functions. This effect of PVAT has been mostly demonstrated in vessels obtained from rats and mice. Thus, the aim of this study was to investigate anti-contractile effect of PVAT surrounding human coronary bypass grafts such as saphenous vein (SV) and internal mammary artery (IMA). Moreover, we aimed to determine the involvement of prostanoids in the anticontractile effect of PVAT. Human SV and IMA preparations were set up in an organ bath. The presence of PVAT in SV and IMA preparations significantly attenuated the contractile response to noradrenaline (NA). Preincubation with indomethacin, a cyclooxygenase inhibitor, increased NA contraction in SV preparations with PVAT. This effect was not observed in IMA preparation with PVAT incubated with indomethacin. The lower measurements of prostaglandin E2 (PGE2) released from PVAT surrounding IMA versus SV supported these effects. In conclusion, our results show that PVAT of SV could attenuate NA-induced contraction by releasing both PGE2 and prostacyclin (PGI2). In contrast to SV, PVAT of IMA exerts its anti-contractile effect independently from prostanoids. These observations suggest that retaining PVAT in human SV and IMA preparations may have potential clinical implications to improve coronary bypass graft patency.