Characterisation and anti-biofilm activity of extracellular polymeric substances from Oceanobacillus iheyensis

• Published in: Carbohydrate polymers 2014-01, Vol.101, p.29-35
• Main Authors: Kavita, Kumari, Singh, Vijay Kumar, Mishra, Avinash, Jha, Bhavanath
• Format: Article
• Language: English
• Subjects: Antibiofilm; Applied sciences; Bacillaceae - chemistry; Bacillaceae - cytology; Bacillaceae - isolation & purification; Biofilm; Biofilms - drug effects; Biofilms - growth & development; Biopolymers - chemistry; Biopolymers - isolation & purification; Biopolymers - pharmacology; Carbohydrates - analysis; EPS; Exact sciences and technology; Exopolymer; Extracellular Space - chemistry; Marine bacteria; Natural polymers; Physicochemistry of polymers; Rheology; Staphylococcus aureus - drug effects; Staphylococcus aureus - physiology; Starch and polysaccharides; Surface-Active Agents - chemistry; Surface-Active Agents - isolation & purification; Surface-Active Agents - pharmacology; Temperature
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2013.08.099

•A marine bacterium (Oceanobacillus iheyensis) was isolated from natural biofilm.•Detailed characterisation of EPS produced by the marine bacterium.•Sugar component of the EPS comprised of mannose (47.78%), glucose (29.71%) and arabinose (22.46%).•EPS showed thermostability, pseudoplastic rheology and emulsifying activity.•EPS exhibited antibiofilm activity against pathogenic strain Staphylococcus aureus. Oceanobacillus iheyensis BK6, isolated from a marine natural biofilm, produced approximately 400mgL−1 extracellular polymeric substances (EPS). FTIR analysis of the EPS revealed different functional groups (halide groups, uronic acid and saccharides). The GCMS showed that the extracellular polysacchrides comprised of mannose (47.78%), glucose (29.71%) and arabinose (22.46%). The molecular mass of the EPS was about 2.14×106Da. It was thermally stable and showed pseudoplastic rheology and emulsifying activity (66.47%). The EPS exhibited antibiofilm activity against a pathogenic strain of Staphylococcus aureus. This is the first report on the characterisation of EPS from the genus Oceanobacillus. The high viscosity, emulsifying properties and antibiofilm activity of EPS make it suitable for potential pharmaceutical and industrial applications.