Functionality of porous starch obtained by amylase or amyloglucosidase treatments

• Published in: Carbohydrate polymers 2014-01, Vol.101, p.837-845
• Main Authors: Dura, A., Błaszczak, W., Rosell, C.M.
• Format: Article
• Language: English
• Subjects: Amylases - metabolism; Applied sciences; Biological and medical sciences; Corn starch; DSC; Enzymatic modification; Exact sciences and technology; Food industries; Fundamental and applied biological sciences. Psychology; Glucan 1,4-alpha-Glucosidase - metabolism; Hydration properties; Hydrolysis; Hydrolysis kinetics; Kinetics; Natural polymers; Physicochemistry of polymers; Porosity; SEM; Starch - chemistry; Starch and polysaccharides; Starch and starchy product industries; Temperature; Zea mays - chemistry
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2013.10.013

•Enzymatic treatment at sub-gelatinization temperature led to porous starch.•α-Amylase or amyloglucosidase action on corn starch was studied.•Pasting and thermal properties of porous starch depended on the enzyme used.•Porous starch was more susceptible to enzymatic digestion. Porous starch is attracting very much attention for its absorption and shielding ability in many food applications. The effect of two different enzymes, fungal α-amylase (AM) or amyloglucosidase (AMG), on corn starch at sub-gelatinization temperature was studied as an alternative to obtain porous starch. Biochemical features, thermal and structural analyses of treated starches were studied. Microscopic analysis of the granules confirmed the enzymatic modification of the starches obtaining porous structures with more agglomerates in the case of AMG treated starches. Several changes in thermal properties and hydrolysis kinetics were observed in enzymatically modified starches. Hydration properties were significantly affected by enzymatic modification being greater influenced by AMG activity, and the opposite trend was observed in the pasting properties. Overall, results showed that enzymatic modification at sub-gelatinization temperatures really offer an attractive alternative for obtaining porous starch granules to be used in a variety of foods applications.