Gene expression changes in initiation and progression of oral squamous cell carcinomas revealed by laser microdissection and oligonucleotide microarray analysis

• Published in: International journal of cancer 2013-02, Vol.132 (3), p.540-548
• Main Authors: Sumino, Jun, Uzawa, Narikazu, Okada, Norihiko, Miyaguchi, Ken, Mogushi, Kaoru, Takahashi, Ken‐Ichiro, Sato, Hiroaki, Michikawa, Chieko, Nakata, Yoshimi, Tanaka, Hiroshi, Amagasa, Teruo
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biomarkers, Tumor - genetics; Cancer; carcinogenesis; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - metabolism; Carcinoma, Squamous Cell - mortality; Carcinoma, Squamous Cell - pathology; Cell Transformation, Neoplastic; Cytokines - biosynthesis; Cytokines - genetics; Disease Progression; Female; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Humans; ISG15; Laser Capture Microdissection; laser microdissection; Male; Medical sciences; microarray; Middle Aged; Mouth Neoplasms - genetics; Mouth Neoplasms - metabolism; Mouth Neoplasms - mortality; Mouth Neoplasms - pathology; Oligonucleotide Array Sequence Analysis; oral; Oral cancer; Otorhinolaryngology. Stomatology; RNA, Messenger - genetics; RNA, Messenger - metabolism; squamous cell carcinoma; Tumors; Ubiquitins - biosynthesis; Ubiquitins - genetics; Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology
• ISSN: 0020-7136; 1097-0215
• DOI: 10.1002/ijc.27702

Oral carcinogenesis is a complex process involving multiple genes. However, the genetic changes involved in this process are not apparent in identical oral squamous cell carcinomas (OSCCs). According to pathological characteristics, samples of normal tissue, oral dysplastic lesions (ODLs), and invasive cancers were obtained from identical OSCCs using laser microdissection (LMD). Large‐scale gene expression profiling was carried out on 33 samples derived from 11 OSCCs. We analyzed genes differentially expressed in normal tissues vs. ODLs and in ODLs vs. invasive tumors and identified 15 candidate genes with continuously increasing or decreasing expression during oral carcinogenesis. One of these genes, ISG15, was chosen for further characterization. Real‐time quantitative reverse transcription‐polymerase chain reaction and immunohistochemical analysis confirmed that ISG15 expression consistently increased during oral tumorigenesis. An ISG15 high‐expression level was significantly associated with poor prognosis (p = 0.027). In addition, patients with high‐expression tumors had a poorer 5‐year survival rate than patients with low expression levels (p = 0.019). In conclusion, we identified 15 genes with continuously increasing or decreasing expression during oral carcinogenesis. One of these, ISG15, is likely to be associated with both dysgenesis and tumorigenesis and may be a potential prognostic marker for oral cancer. What's new? Oral epithelial dysplasia is a potentially precancerous lesion diagnosed histopathologically. More accurate markers predicting progression to invasive cancer are needed to enable better diagnosis of such lesions and more appropriate selection of aggressive treatment and closer follow‐up. This is the first study to demonstrate gene expression changes during oral carcinogenesis using normal epithelial, premalignant, and carcinoma cells from the same oral cancer. The study also examined ISG15 expression at both mRNA and protein level in oral pre‐malignant lesions and invasive cancers, demonstrating the association between ISG15 expression status and clinicopathological factors and patients survival.