Evaluation of bioremediation effectiveness on crude oil-contaminated sand

• Published in: Chemosphere (Oxford) 2005-05, Vol.59 (6), p.845-852
• Main Authors: Kim, Sang-Jin, Choi, Dong Hyuk, Sim, Doo Suep, Oh, Young-Sook
• Format: Article
• Language: English
• Subjects: Applied sciences; beach soils; beaches; biodegradation; Biodegradation of pollutants; Biodegradation, Environmental; biological activity in soil; Biological and medical sciences; Bioremediation; Biotechnology; carbon dioxide; Carbon Dioxide - analysis; Carbon Dioxide - metabolism; CO 2 evolution rate; Corynebacterium; Corynebacterium - metabolism; Decontamination. Miscellaneous; Environment and pollution; ETS activity; Exact sciences and technology; Fertilizers; Fundamental and applied biological sciences. Psychology; gas production (biological); hydrocarbons; Industrial applications and implications. Economical aspects; nitrogen; Norhopane ratio; nutrient availability; Nutrients; Oil degradation rate; Oil-degrading microorganisms; petroleum; Petroleum - metabolism; phosphorus; Pollution; Saccharomycetales; sand; Silicon Dioxide; Soil and sediments pollution; Sphingomonas; Sphingomonas - metabolism; Surfactant; Yarrowia; Yarrowia - metabolism
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2004.10.058

A treatability study was conducted using sea sand spiked with 3% or 6% (w/w) of Arabian light crude oil to determine the most effective bioremediation strategies for different levels of contamination. The sea sand used in the study was composed of gravel (0.1%), sand (89.0%), and silt and clay (10.9%). The water content of the sea sand was adjusted to 12.6% (w/w) for the study. Different combinations of the following treatments were applied to the sand in biometer flasks: the concentration of oil (3% or 6%), the concentration of a mixture of three oil-degrading microorganisms ( Corynebacterium sp. IC-10, Sphingomonas sp. KH3-2 and Yarrowia sp. 180, 1 × 10 6 or 1 × 10 8 cells g −1 sand), the concentration of the surfactant Tween 80 (1 or 10 times the critical micelle concentration), and the addition of SRIF in a C:N:P ratio of 100:10:3. Three biometer flasks per combination of experimental conditions were incubated, and the performance of each treatment was examined by monitoring CO 2 evolution, microbial activity, and oil degradation rate. The results suggest that the addition of inorganic nutrients accelerated the rate of CO 2 evolution by a factor of 10. The application of oil-degrading microorganisms in a concentration greater than that of the indigenous population clearly increased biodegradation efficiency. The application of surfactant slightly enhanced the oil degradation rate in the contaminated sand treated with the higher concentration of oil-degrading microorganisms. The initial CO 2 evolution rate was shown to efficiently evaluate the treatability test by providing significant data within a short period, which is critical for the rapid determination of the appropriate bioremediation approach. The measurements of microbial activity and crude oil degradation also confirmed the validity of the CO 2 evolution rate as an appropriate criterion.