The effect of ozone on progression or regression of artificial caries-like enamel lesions in vitro

• Published in: Journal of dentistry 2014-02, Vol.42 (2), p.167-174
• Main Authors: Tahmassebi, J.F, Chrysafi, N, Duggal, M.S
• Format: Article
• Language: English
• Subjects: Caries; Cariostatic Agents - therapeutic use; Dental caries; Dental Caries - pathology; Dental Caries - prevention & control; Dental Enamel - drug effects; Dental Enamel - ultrastructure; Dentin - drug effects; Dentin - ultrastructure; Dentistry; Disease Progression; Enamel; Fluorescence; Fluorides; Fluorides - therapeutic use; Hardness; Humans; Hydrogen-Ion Concentration; Materials Testing; Microhardness; Observer Variation; Orthodontics; Oxidants, Photochemical - therapeutic use; Ozone; Ozone - therapeutic use; Reducing Agents - therapeutic use; Saliva, Artificial - chemistry; Single-Blind Method; Studies; Temperature; Tooth Remineralization - methods
• ISSN: 0300-5712; 1879-176X
• DOI: 10.1016/j.jdent.2013.11.011

Abstract Objective This study investigated the effect of ozone on the progression or regression of artificial caries-like lesions on enamel following pH cycling conditions in vitro. Methods A randomized, single blind, four legs design was used. 20 full thickness enamel slabs were allocated to each of the four groups which were: Fluoride free toothpaste (control); ozone alone; Reductant/Patient Kit alone and a combination of both ozone/Reductant/Patient Kit. Artificial lesions were created and subjected to the pH cycling regime for a 14 days period. Assessments were carried out before and after the pH cycling on the slabs using the microhardness testing and Quantitative Light-induced Fluorescence (QLF). Results Statistical significant difference were found in the percentage change of enamel microhardness before and after pH cycling between ozone/Reductant/Patient Kit group and all the other three groups of the study, as well as between Reductant/Patient Kit group and control. There was a statistical significant difference in the change of size and severity of the lesion (Δ Q ) between all the three regimes tested and the control with a trend favouring ozone/Reductant/Patient Kit group. Conclusions In our model, it appeared that ozone treatment alone is not effective in protecting the enamel against demineralisation or promoting remineralisation, unless combined with the Reductant/Patient Kit, which contain high levels of fluoride.