Immunohistochemical analysis of mast cell infiltrates and microvessel density in oral squamous cell carcinoma

The aim of the study was to evaluate mast cell concentration and microvessel density in perilesional and intralesional regions of oral squamous cell carcinoma (OSCC) and furthermore to assess the possible relationship between the above-mentioned parameters. Paraffin-embedded specimens from 47 cases...

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Bibliographic Details
Published in:Polish journal of pathology 2013-12, Vol.64 (4), p.276-280
Main Authors: Pyziak, L, Stasikowska-Kanicka, O, Danilewicz, M, Wągrowska-Danilewicz, M
Format: Article
Language:English
Subjects:
Angiogenesis
Antibodies
Cancer
Carcinoma, Squamous Cell - blood supply
Carcinoma, Squamous Cell - metabolism
Carcinoma, Squamous Cell - pathology
Cloning
Female
Growth factors
Humans
Immunohistochemistry
Male
Mast Cells - metabolism
Mast Cells - pathology
Microvessels - metabolism
Microvessels - pathology
Middle Aged
Mouth - blood supply
Mouth Neoplasms - blood supply
Mouth Neoplasms - metabolism
Mouth Neoplasms - pathology
Neovascularization, Pathologic - pathology
Platelet Endothelial Cell Adhesion Molecule-1 - metabolism
Tryptases - metabolism
Tumor necrosis factor-TNF
Tumorigenesis
Tumors
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Summary:The aim of the study was to evaluate mast cell concentration and microvessel density in perilesional and intralesional regions of oral squamous cell carcinoma (OSCC) and furthermore to assess the possible relationship between the above-mentioned parameters. Paraffin-embedded specimens from 47 cases of OSCC and 12 cases of normal mucosa were investigated immunohistochemically with anti-CD-31 antibody to stain microvessels and anti-tryptase antibody to visualize mast cells. The degree of vascularization and mast cell infiltration was measured with an image analysis system. The study revealed considerably increased microvessel density and mast cell abundance in intralesional and perilesional regions of OSCCs in comparison to normal mucosa. There was a significant positive correlation between microvessel density and mast cell concentration in both localizations of OSCCs (p < 0.02, p < 0.001, respectively), whereas a comparable correlation was not observed in normal mucosa. The obtained results suggest that mast cells play an important role in the regulation of angiogenesis in OSCC, although there are aspects of their activity of potential diagnostic and therapeutic significance which require further research.
ISSN:1233-9687
2084-9869
DOI:10.5114/PJP.2013.39336