Quorum sensing inhibitory activities of surface immobilized antibacterial dihydropyrrolones via click chemistry

Abstract Device-related infection remains a major barrier to the use of biomaterial implants as life-saving devices. This study aims to examine the effectiveness and mechanism of action of surface attached dihydropyrrolones (DHPs), a quorum sensing (QS) inhibitor, against bacterial colonization. DHP...

Full description

Saved in:
Bibliographic Details
Published in:Biomaterials 2014-02, Vol.35 (7), p.2336-2345
Main Authors: Ho, Kitty K.K, Chen, Renxun, Willcox, Mark D.P, Rice, Scott A, Cole, Nerida, Iskander, George, Kumar, Naresh
Format: Article
Language:English
Subjects:
Advanced Basic Science
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Antibacterial
Click Chemistry
Dentistry
Dihydropyrrolone
Microscopy, Confocal
Pseudomonas
Pseudomonas aeruginosa - drug effects
Pyrroles - chemistry
Pyrroles - pharmacology
Quorum Sensing
Spectrum Analysis - methods
Staphylococcus
Staphylococcus aureus - drug effects
Surface modification
Surface Properties
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Device-related infection remains a major barrier to the use of biomaterial implants as life-saving devices. This study aims to examine the effectiveness and mechanism of action of surface attached dihydropyrrolones (DHPs), a quorum sensing (QS) inhibitor, against bacterial colonization. DHPs were covalently attached on glass surfaces via copper-catalyzed azide-alkyne 1,3-dipolar cycloaddition (CuAAC) click reaction. The covalent attachment of DHP surfaces was confirmed by X-ray photoelectron spectroscopy (XPS) and contact angle measurements, and the antimicrobial efficacy of the DHP coatings was assessed by confocal laser scanning microscopy (CLSM) and image analysis. The results demonstrated that covalently bound DHP compounds are effective in reducing the adhesion by up to 97% ( p  
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2013.11.072