Isolation and characterization of a new Staphylococcus epidermidis broad-spectrum bacteriophage

Staphylococcus epidermidis is considered an important nosocomial pathogen, being very tolerant to the host immune system and antibiotherapy, particularly when in biofilms. Due to its high resistance, alternative antimicrobial strategies are under development. The use of bacteriophages is seen as an...

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Bibliographic Details
Published in:Journal of general virology 2014-02, Vol.95 (PART 2), p.506-515
Main Authors: Melo, L. D. R., Sillankorva, Sanna, Ackermann, H. W., Kropinski, A. M., Azeredo, Joana, Cerca, Nuno
Format: Article
Language:English
Subjects:
DNA, Viral - chemistry
DNA, Viral - genetics
Genome, Viral
Host Specificity
Humans
Molecular Sequence Data
Myoviridae - growth & development
Myoviridae - isolation & purification
Myoviridae - physiology
Myoviridae - ultrastructure
Science & Technology
Sequence Analysis, DNA
Sequence Homology
Staphylococcus epidermidis - virology
Staphylococcus Phages - growth & development
Staphylococcus Phages - isolation & purification
Staphylococcus Phages - physiology
Staphylococcus Phages - ultrastructure
Synteny
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Summary:Staphylococcus epidermidis is considered an important nosocomial pathogen, being very tolerant to the host immune system and antibiotherapy, particularly when in biofilms. Due to its high resistance, alternative antimicrobial strategies are under development. The use of bacteriophages is seen as an important strategy to combat pathogenic organisms. In this study, a S. epidermidis myovirus, SEP1, was isolated and characterized. The genome of this phage was sequenced and shown to be peripherally related to the Twortlikevirus genus. However when compared to other phages of this genus, it showed DNA sequence identities no greater than 58.2%. As opposed to other polyvalent viruses of the Twortlikevirus genus, SEP1 is highly specific to S. epidermidis strains. The good infectivity showed by this phage as well as its high lytic spectrum suggests that it might be a good candidate for therapeutic studies. L. D. R. M. and S. S. acknowledge Fundac¸a˜o para a Cieˆncia e a Tecnologia grants SFRH/BD/66166/2009 and SFRH/BPD/48803/2008, respectively. We thank Gabriele Bierbaum (Institute of Medical Microbiology, Immunology and Parasitology, University of Bonn, Bonn, Germany) for providing some strains.
ISSN:0022-1317
0022-1317
1465-2099
DOI:10.1099/vir.0.060590-0