cDNA Cloning of Human Liver Monoamine Oxidase A and B: Molecular Basis of Differences in Enzymatic Properties

The monoamine oxidases play a vital role in the metabolism of biogenic amines in the central nervous system and in peripheral tissues. Using oligonucleotide probes derived from three sequenced peptide fragments, we have isolated cDNA clones that encode the A and B forms of monoamine oxidase and have...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1988-07, Vol.85 (13), p.4934-4938
Main Authors: Alfred W. J. Bach, Lan, Nancy C., Johnson, Deborah L., Abell, Creed W., Bembenek, Michael E., Kwan, Sau-Wah, Seeburg, Peter H., Shih, Jean C.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Base Sequence
Biological and medical sciences
Biotechnology
Complementary DNA
DNA - genetics
Enzymes
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Humans
Liver
Liver - enzymology
Messenger RNA
Methods. Procedures. Technologies
Molecular cloning
Molecular Sequence Data
Molecular Weight
Monoamine Oxidase - genetics
Nucleotides
Oligonucleotide probes
Oxidases
Proteins
RNA
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Summary:The monoamine oxidases play a vital role in the metabolism of biogenic amines in the central nervous system and in peripheral tissues. Using oligonucleotide probes derived from three sequenced peptide fragments, we have isolated cDNA clones that encode the A and B forms of monoamine oxidase and have determined the nucleotide sequences of these cDNAs. Comparison of the deduced amino acid sequences shows that the A and B forms have subunit molecular weights of 59,700 and 58,800, respectively, and have 70% sequence identity. Both sequences contain the pentapeptide Ser-Gly-Gly-Cys-Tyr, in which the obligatory cofactor FAD is covalently bound to cysteine. Based on differences in primary amino acid sequences and RNA gel blot analysis of mRNAs, the A and B forms of monoamine oxidase appear to be derived from separate genes.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.85.13.4934