Phytosulfokine alpha enhances microspore embryogenesis in both triticale and wheat

Isolated microspore culture (IMC) has been used to develop doubled haploid lines and to generate homozygous lines in a single generation for varietal development. Phytosulfokine has been previously used in promoting cell growth and embryo development in various systems. In this study, phytosulfokine...

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Published in:Plant cell, tissue and organ culture tissue and organ culture, 2014, Vol.116 (1), p.125-130
Main Authors: Asif, Muhammad, Eudes, François, Randhawa, Harpinder, Amundsen, Eric, Spaner, Dean
Format: Article
Language:English
Subjects:
Albinism
albino
Biomedical and Life Sciences
Cell culture
cell growth
coculture
Cultivars
Embryogenesis
Embryonic growth stage
Embryos
Life Sciences
Nursing
Ovaries
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
Research Note
Triticale
Triticum aestivum
Wheat
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container_issue 1
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container_title Plant cell, tissue and organ culture
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creator Asif, Muhammad
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Amundsen, Eric
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description Isolated microspore culture (IMC) has been used to develop doubled haploid lines and to generate homozygous lines in a single generation for varietal development. Phytosulfokine has been previously used in promoting cell growth and embryo development in various systems. In this study, phytosulfokine alpha (PSK-α) supplemented IMC induction medium was evaluated in triticale and wheat cultivars, and the production of embryo-like structures (ELS), green and albino plants were recorded. In addition, the contribution of ovary co-culture was also evaluated in IMC on a NPB99 + 10F induction medium. Over a range of concentrations up to 10⁻⁷M, PSK-α yielded more ELS and green plants in wheat and triticale cultivars at the highest dose, when compared to the control. It also minimized albinism in wheat cultivars, but not in triticale. Interestingly, 10⁻⁷M PSK-α also supported the formation of a large number of embryos and a few green plants in the absence of nursing ovaries.
doi_str_mv 10.1007/s11240-013-0379-y
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source Springer Nature
subjects Albinism
albino
Biomedical and Life Sciences
Cell culture
cell growth
coculture
Cultivars
Embryogenesis
Embryonic growth stage
Embryos
Life Sciences
Nursing
Ovaries
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
Research Note
Triticale
Triticum aestivum
Wheat
title Phytosulfokine alpha enhances microspore embryogenesis in both triticale and wheat
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