A novel fed-batch process based on the biology of Aurantiochytrium sp. KRS101 for the production of biodiesel and docosahexaenoic acid

• Published in: Bioresource technology 2013-05, Vol.135, p.269-274
• Main Authors: Kim, Kyochan, Jung Kim, Eun, Ryu, Byung-Gon, Park, Soojung, Choi, Yoon-E, Yang, Ji-Won
• Format: Article
• Language: English
• Subjects: Aurantiochytrium sp; Batch Cell Culture Techniques - methods; Biodiesel; Biofuels; Biology; Biomass; Carbon; Carbon - pharmacology; cell division; cell growth; Cultivation; developmental stages; Docosahexaenoic acid; Docosahexaenoic Acids - biosynthesis; Enlargement; Glucose - pharmacology; Mathematical analysis; Modified fed-batch; nitrogen; Nitrogen - pharmacology; nutrients; palmitic acid; Palmitic Acid - metabolism; Salinity; Sodium Chloride - pharmacology; Stramenopiles - cytology; Stramenopiles - drug effects; Stramenopiles - growth & development; Stramenopiles - metabolism; Yield coefficients
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2012.10.139

► Aurantiochytrium sp. is of considerable interest for docosahexaenoic acid (DHA), and biodiesel. ► Aurantiochytrium biology was investigated, particularly yield coefficients to nutrients. ► Nitrogen is mostly responsible for early stage of cell growth or division. ► Carbon is rather necessary for later stage of cell development. ► With the yield coefficients, modified fed-batch cultivation was successfully performed. The biology of Aurantiochytrium sp. KRS101 was thoroughly investigated to enhance its production of biodiesel and docosahexaenoic acid (DHA). Nutrients and salinity were optimized to prevent biomass loss due to cell rupture. Calculation of yield coefficients showed that nitrogen was mostly responsible for the early stage of cell growth or division, whereas carbon was necessary for the entire process of cell development, particularly cell enlargement during late stages. Using these distinctive yield coefficients, a modified fed-batch cultivation method was designed, resulting in increases in palmitic acid (PA) and DHA production of up to 137% and 29%, respectively. This modified fed-batch cultivation method, using appropriate supplies of nitrogen and carbon, may improve the yields of PA and DHA, thus expanding the biotechnological applications of Aurantiochytrium sp. KRS101.